Immunohistochemical localisation of PDE5 in Leydig and myoid cells of prepuberal and adult rat testis

Expression of phosphodiesterase 5 (PDE5) in the rat testis at several pre and postnatal developmental stages was investigated by immunohistochemistry. The enzyme was localised in vascular smooth muscle cells, as well as in Leydig and peritubular cells. The latter were identified as myoid, based on t...

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Bibliographic Details
Published in:Histochemistry and cell biology 2005-11, Vol.124 (5), p.401-407
Main Authors: Scipioni, Angela, Stefanini, Stefania, Santone, Rocco, Giorgi, Mauro
Format: Article
Language:English
Subjects:
3',5'-Cyclic-GMP Phosphodiesterases - metabolism
Actins - metabolism
Animals
Animals, Newborn
Biomarkers - metabolism
Blotting, Western
Cell Fractionation
Cyclic Nucleotide Phosphodiesterases, Type 5
Fetal Development - physiology
Fluorescent Antibody Technique, Indirect
Gestational Age
Immunoenzyme Techniques
Leydig Cells - cytology
Leydig Cells - enzymology
Male
Muscle, Smooth, Vascular - cytology
Muscle, Smooth, Vascular - enzymology
Rats
Rats, Wistar
Seminiferous Tubules - cytology
Seminiferous Tubules - metabolism
Testis - embryology
Testis - enzymology
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Summary:Expression of phosphodiesterase 5 (PDE5) in the rat testis at several pre and postnatal developmental stages was investigated by immunohistochemistry. The enzyme was localised in vascular smooth muscle cells, as well as in Leydig and peritubular cells. The latter were identified as myoid, based on their immunoreactivity to desmin and alpha-smooth muscle actin. The presence of PDE5 in myoid cells was confirmed by Western blot analysis and immunohistochemistry performed on highly purified cell fractions, obtained from 16-day-old rats. The expression of PDE5 in these somatic cells of rat testis is discussed in view of the roles played by cGMP signal transduction pathways in the mammalian male reproductive function.
ISSN:0948-6143
1432-119X
DOI:10.1007/s00418-005-0057-1