Accumulation of polyphosphates and expression of high molecular weight exopolyphosphatase in the yeast Saccharomyces cerevisiae

The effect of cultivation time and concentration of inorganic phosphate (P(i)) in the culture medium on the accumulation of polyphosphates (polyP) and the activity of two cytosolic exopolyphosphatases of the yeast Saccharomyces cerevisiae was studied: an exopolyphosphatase of 40 kD encoded by PPX1 a...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2005-09, Vol.70 (9), p.980-985
Main Authors: Kulakovskaya, T V, Andreeva, N A, Trilisenko, L V, Suetin, S V, Vagabov, V M, Kulaev, I S
Format: Article
Language:English
Subjects:
Accumulation
Acid Anhydride Hydrolases - metabolism
Brewer's yeast
Culture Media
Cytoplasm
Cytosol
Cytosol - enzymology
Molecular Weight
Permeability
Phosphates
Phosphates - chemistry
Phosphates - metabolism
Phosphazo compounds
Polyphosphates - chemistry
Polyphosphates - metabolism
Proteins
Saccharomyces cerevisiae
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - metabolism
Spheroplasts - enzymology
Spheroplasts - metabolism
Time Factors
Yeast
Yeast fungi
Yeasts
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Summary:The effect of cultivation time and concentration of inorganic phosphate (P(i)) in the culture medium on the accumulation of polyphosphates (polyP) and the activity of two cytosolic exopolyphosphatases of the yeast Saccharomyces cerevisiae was studied: an exopolyphosphatase of 40 kD encoded by PPX1 and a high molecular weight exopolyphosphatase encoded by another gene. Depletion of polyP in the cells on P(i) starvation is a signal factor for the accumulation of polyP after the subsequent addition of 5-20 mM P(i) and glucose to the cells or spheroplasts. A high activity of both exopolyphosphatases does not prevent the accumulation of polyP. The expression of the high molecular weight exopolyphosphatase is due to the acceleration of metabolism in cells that have reached the stage of growth deceleration on the addition of P(i) and glucose or complete culture medium. This process may occur independently from the accumulation of polyP. The activity of exopolyphosphatase PPX1 depends less on the mentioned factors, decreasing 10-fold only under conditions of phosphate surplus at the stationary growth stage.
ISSN:0006-2979
1608-3040
0320-9725
DOI:10.1007/s10541-005-0212-2