Differential expression of fungal genes at preinfection and mycorrhiza establishment between Terfezia boudieri isolates and Cistus incanus hairy root clones

• Changes in gene expression by isolates of Terfezia boudieri during mycorrhization with Cistus incanus hairy roots were followed. • Four fungus-hairy root clone combinations were cultivated under two sets of conditions, in which the root and the fungus were separated by a cellophane sheet or were a...

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Bibliographic Details
Published in:The New phytologist 2006-01, Vol.171 (4), p.837-846
Main Authors: Zaretsky, Marianna, Sitrit, Yaron, Mills, David, Roth-Bejerano, Nurit, Kagan-Zur, Varda
Format: Article
Language:English
Subjects:
Ascomycota - genetics
Biological and medical sciences
cDNA–AFLP (amplified fragment length polymorphism)
Cistus
Cistus - microbiology
Complementary DNA
Ectomycorrhizas
Endomycorrhizas
Fundamental and applied biological sciences. Psychology
Fungal genes
Fungal Proteins - genetics
Gene expression
Gene Expression Regulation, Fungal
Genes, Fungal - genetics
Mycorrhizae - genetics
mycorrhizal type determination
Mycorrhizas
Parasitism and symbiosis
Plant physiology and development
Plant roots
Plant Roots - microbiology
Plants
Protein metabolism
reverse Northern
RNA
Symbiosis
Terfezia boudieri
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Summary:• Changes in gene expression by isolates of Terfezia boudieri during mycorrhization with Cistus incanus hairy roots were followed. • Four fungus-hairy root clone combinations were cultivated under two sets of conditions, in which the root and the fungus were separated by a cellophane sheet or were allowed physical contact. One of the combinations produced endomycorrhizas, the other three solely ectomycorrhizas. Fragments isolated by cDNA-AFLP analysis from cellophane-separated cultures (preinfection) were used to identify differentially expressed genes by reverse Northern analysis. • Genes showing no homology to known sequences constituted the largest group under both growth conditions. Some fungal genes were expressed transiently, while others exhibited altered expression patterns as conditions changed from individually growing through the preinfection stage to mycorrhizas. Genes expressed exclusively under combinations allowing either ectomycorrhiza or endomycorrhiza under a particular condition were detected. • Our results point, for the first time, to some of the genes that might be involved in determining the type of association that will be formed: ecto- or endomycorrhiza.
ISSN:0028-646X
1469-8137
DOI:10.1111/j.1469-8137.2006.01791.x