Cholinergic regulation of the central nucleus of the amygdala in rats: Effects of local microinjections of cholinomimetics and cholinergic antagonists on arousal and sleep

• Published in: Neuroscience 2006-01, Vol.141 (4), p.2167-2176
• Main Authors: Sanford, L.D., Yang, L., Tang, X., Dong, E., Ross, R.J., Morrison, A.R.
• Format: Article
• Language: English
• Subjects: amygdala; Amygdala - drug effects; Animals; Biological and medical sciences; carbachol; Carbachol - pharmacology; Cholinergic Agents - pharmacology; Electroencephalography - methods; Fundamental and applied biological sciences. Psychology; Male; mecamylamine; Microinjections - methods; neostigmine; Neostigmine - pharmacology; Polysomnography - methods; Rats; Rats, Wistar; scopolamine; sleep; Sleep - drug effects; Sleep. Vigilance; Vertebrates: nervous system and sense organs; Wakefulness - drug effects
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2006.05.064

The amygdala has emerged as an important forebrain modulator of arousal. Acetylcholine plays a role in the regulation of sleep and wakefulness, particularly rapid eye movement sleep (REM). The major cholinergic input to the amygdala comes from the basal forebrain, a region primarily linked to wakefulness. We examined sleep and the encephalogram for 8 h following bilateral microinjections into the central nucleus of the amygdala (CNA) of the cholinergic agonist, carbachol (CARBL: 0.3 μg; CARBH: 3.0 μg), the acetylcholinesterase inhibitor, neostigmine (NEOL: 0.3 μg; NEOH: 3.0 μg), the muscarinic antagonist, scopolamine (SCOL: 0.3 μg; SCOH: 1.0 μg), the nicotinic antagonist, mecamylamine (MECL: 0.3 μg; MECH: 1.0 μg) and saline (SAL, 0.2 μl) alone. Both doses of CARB and NEO significantly reduced REM, but did not significantly alter non-rapid eye movement sleep (NREM). Both doses of SCO significantly increased NREM, and SCOH also produced an initial increase in REM followed by a significant decrease. CARBH and NEOH decreased REM electroencephalogram (EEG) power in the 5.5–10 Hz band, and NEOL and NEOH decreased NREM EEG power in the 0.5–5.0 Hz band. CARBL decreased waking EEG power in the 0.5–5.0 Hz band, and NEOH decreased waking EEG power in the 5.0–10.0 Hz band. Both doses of SCO significantly increased waking EEG power in the 5.5–10.0 Hz band. Compared with SAL, MEC did not significantly alter sleep or EEG power. The reduction of REM by CARB and NEO and the alteration of sleep by SCO indicate that cholinergic regulation of the amygdala is involved in the control of arousal in rodents. In contrast, CARB microinjections into CNA increase REM in cats, though the reasons for the species difference are not known. The results are discussed in the context of anatomical inputs and species differences in the cholinergic regulation of CNA.