Visualization of infectious pancreatic necrosis virus (IPNV) particles labeled with fluorescent probes

Infectious pancreatic necrosis virus (IPNV) particles were labeled with SYBR Green I or a monoclonal antibody and FITC-conjugated secondary antibody and examined in a fluorescence microscope. Labeled viral particles were visualized in a narrow range of pixels. Comparing IPNV particles with fluoresce...

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Bibliographic Details
Published in:Journal of virological methods 2005-12, Vol.130 (1), p.140-144
Main Authors: Osorio, Manuel, Espinoza, Juan Carlos, Kuznar, Juan
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Line
Fundamental and applied biological sciences. Psychology
Infectious pancreatic necrosis virus
Infectious pancreatic necrosis virus - isolation & purification
Microbiology
Microscopy, Fluorescence
Organic Chemicals
Phage T4
Staining and Labeling - methods
Techniques used in virology
Virology
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Description
Summary:Infectious pancreatic necrosis virus (IPNV) particles were labeled with SYBR Green I or a monoclonal antibody and FITC-conjugated secondary antibody and examined in a fluorescence microscope. Labeled viral particles were visualized in a narrow range of pixels. Comparing IPNV particles with fluorescent phage T4 virions, the former, as expected, were seen smaller in size. The method allows the rapid and accurate counting of viral particles both on filters and bound to the cell surface. In addition, IPNV particles can be specifically enumerated in the presence of other virions and the ratio between physical particles and virus infectivity can be easily calculated as well.
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2005.06.007