A death round affecting a young compartmentalized mycelium precedes aerial mycelium dismantling in confluent surface cultures of Streptomyces antibioticus

1 Area de Microbiología, Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, Julián Clavería s/n, 33006 Oviedo, Spain 2 Laboratorio de Proteómica, Centro Nacional de Biotecnología, Cantoblanco, 28049 Madrid, Spain Correspondence Jesús Sánchez jsm{at}fq.uniovi.es Developm...

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Published in:Microbiology (Society for General Microbiology) 2005-11, Vol.151 (11), p.3689-3697
Main Authors: Manteca, Angel, Fernandez, Marisol, Sanchez, Jesus
Format: Article
Language:English
Subjects:
Bacteriology
Biogenesis of cell structures, supramolecular organization
Biological and medical sciences
Cell Membrane Permeability
Culture Media
Fundamental and applied biological sciences. Psychology
Microbiology
Microscopy, Confocal
Microscopy, Fluorescence
Mycelium - growth & development
Mycelium - ultrastructure
Organic Chemicals
Propidium
Streptomyces antibioticus
Streptomyces antibioticus - growth & development
Streptomyces antibioticus - ultrastructure
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Summary:1 Area de Microbiología, Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, Julián Clavería s/n, 33006 Oviedo, Spain 2 Laboratorio de Proteómica, Centro Nacional de Biotecnología, Cantoblanco, 28049 Madrid, Spain Correspondence Jesús Sánchez jsm{at}fq.uniovi.es Development-associated cell-death processes were investigated in detail during the growth and differentiation of Streptomyces antibioticus ATCC 11891 on confluent surface cultures, by using fluorescent viability probes, membrane and activity fluorescence indicators, and electron microscopy analysis. A previously unsuspected complexity was revealed, namely the presence of a very young compartmentalized mycelium that dies following an orderly pattern, leaving alternating live and dead segments in the same hypha. This death round is followed by the growth of a second mycelium which develops rapidly from the live segments of the first mycelium and dies massively in a second death round, which extends over the phases of aerial mycelium formation and sporulation. Abbreviations: CFDA, carboxyfluorescein diacetate; CLSM, confocal laser-scanning fluorescence microscopy; FM 4-64, N -(3-triethylammoniumpropyl)-4-( p -diethylaminophenyl-hexatrienyl) pyridinium dibromide; PI, propidium iodide; WGA, wheat germ agglutinin
ISSN:1350-0872
1465-2080
DOI:10.1099/mic.0.28045-0