Discovery of proteins related to coronary artery disease using industrial-scale proteomics analysis of pooled plasma

Relating a disease state to an entire population of proteins provides an opportunity to gain new insights into a disease. Male populations of 53 patients with angiographic coronary artery disease and 53 control subjects without coronary disease from the Duke Databank for Cardiovascular Disease were...

Full description

Saved in:
Bibliographic Details
Published in:The American heart journal 2006-09, Vol.152 (3), p.478-485
Main Authors: Donahue, Mark P., Rose, Keith, Hochstrasser, Denis, Vonderscher, Jacky, Grass, Peter, Chibout, Salah-Dine, Nelson, Charlotte L., Sinnaeve, Peter, Goldschmidt-Clermont, Pascal J., Granger, Christopher B.
Format: Article
Language:English
Subjects:
Acute coronary syndromes
Adult
Aged
Bioinformatics
Biological and medical sciences
Biomarkers
Biomarkers - blood
Blood Proteins - isolation & purification
Cardiology. Vascular system
Cardiovascular disease
Coronary Artery Disease - blood
Coronary Artery Disease - diagnosis
Coronary Artery Disease - pathology
Coronary heart disease
Coronary vessels
Diabetes
Genomes
Heart
Heart attacks
Heart failure
Humans
Insulin-like growth factors
Intubation
Kinases
Male
Medical sciences
Middle Aged
Molecular weight
Peptides
Plasma
Proteins
Proteomics
Proteomics - methods
Risk Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Relating a disease state to an entire population of proteins provides an opportunity to gain new insights into a disease. Male populations of 53 patients with angiographic coronary artery disease and 53 control subjects without coronary disease from the Duke Databank for Cardiovascular Disease were established and matched for age and race as well as extremes of risk factors. Major plasma protein abnormalities were excluded. Plasma samples of each group were pooled to make large volumes (6 L each) to identify low-abundance proteins. After removal of albumin as well as immunoglobulins and enrichment of smaller proteins (
ISSN:0002-8703
1097-6744
DOI:10.1016/j.ahj.2006.03.007