Comparison of the lipid composition of porcine buccal and esophageal permeability barriers

Pig esophageal mucosa has been shown to be a useful and practical substitute for buccal mucosa in in vitro permeability studies in that it offers a larger surface area and it is much easier to prepare. Further, the tissues demonstrate similar histological characteristics. The objectives of this work...

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Bibliographic Details
Published in:Archives of oral biology 2005-12, Vol.50 (12), p.981-987
Main Authors: Diaz-del Consuelo, Isabel, Jacques, Yves, Pizzolato, Gian-Paolo, Guy, Richard H., Falson, Françoise
Format: Article
Language:English
Subjects:
AMD-HPTLC
Animals
Buccal epithelium
Ceramides - analysis
Cholesterol - analysis
Chromatography, Liquid
Dentistry
Esophageal epithelium
Esophagus
Fentanyl - pharmacokinetics
Membrane lipids
Membrane Lipids - analysis
Mouth Mucosa - chemistry
Mouth Mucosa - metabolism
Mucous Membrane - chemistry
Mucous Membrane - metabolism
Permeability
Phospholipids - analysis
Swine
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Summary:Pig esophageal mucosa has been shown to be a useful and practical substitute for buccal mucosa in in vitro permeability studies in that it offers a larger surface area and it is much easier to prepare. Further, the tissues demonstrate similar histological characteristics. The objectives of this work were to characterize the lipid composition of the esophageal mucosa, to compare it to that of the buccal tissue, and to correlate lipid composition with the membranes’ permeability to fentanyl. The major lipid classes of buccal and esophageal epithelia were separated and analysed by automated multiple development high-performance thin-layer chromatography (AMD-HPTLC). The two epithelia presented a very similar lipid pattern. In general, there were more polar lipids than non-polar; glycosylceramides were relatively abundant whereas the amount of ceramides present was very small. The flux of fentanyl applied as the citrate in aqueous solution was comparable across the buccal and esophageal barriers. Lipid extraction provoked a significant increase in permeability. In conclusion, this research confirms the suitability of the esophageal mucosa as a model for buccal permeability studies.
ISSN:0003-9969
1879-1506
DOI:10.1016/j.archoralbio.2005.04.008