ER Stress Pathway Involving CHOP Is Activated in the Lungs of LPS-Treated Mice

CHOP is a C/EBP family transcription factor involved in endoplasmic reticulum (ER) stress-mediated apoptosis. To determine if the ER stress pathway is involved in the pathogenesis of LPS-treated mouse lung injury, mice were given lipopolysaccharide (LPS) intraperitoneally. The mRNAs for activating t...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 2005-10, Vol.138 (4), p.501-507
Main Authors: Endo, Motoyoshi, Oyadomari, Seiichi, Suga, Moritaka, Mori, Masataka, Gotoh, Tomomi
Format: Article
Language:English
Subjects:
Activating Transcription Factor 4 - biosynthesis
Animals
apoptosis
Apoptosis - physiology
ATF4
C/EBP homologous protein
CHOP
Disease Models, Animal
DNA-Binding Proteins - biosynthesis
endoplasmic reticulum
Endoplasmic Reticulum - metabolism
endoplasmic reticulum stress
Humans
inflammation
lipopolysaccharide
Lipopolysaccharides - pharmacology
Lung - drug effects
Lung - metabolism
Mice
Mice, Knockout
nitric oxide
Nuclear Proteins - biosynthesis
Regulatory Factor X Transcription Factors
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
RNA, Messenger - drug effects
Shock, Septic - metabolism
Transcription Factor CHOP - genetics
Transcription Factor CHOP - metabolism
Transcription Factors
Tumor Cells, Cultured
X-Box Binding Protein 1
XBP1
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:CHOP is a C/EBP family transcription factor involved in endoplasmic reticulum (ER) stress-mediated apoptosis. To determine if the ER stress pathway is involved in the pathogenesis of LPS-treated mouse lung injury, mice were given lipopolysaccharide (LPS) intraperitoneally. The mRNAs for activating transcription factor (ATF) 4 and X-box binding protein (XBP) 1, transcriptional activators of the CHOP gene, and that for CHOP were induced by or after the LPS treatment. Apoptosis induced by LPS treatment was suppressed in the lungs of Chop-knockout mice. Overexpression of CHOP induced apoptosis in a lung cancer-derived cell line. These results suggest that the ER stress pathway, involving CHOP, is activated and plays a role in the pathogenesis of septic shock lung.
ISSN:0021-924X
1756-2651
DOI:10.1093/jb/mvi143