Repression of GR-Mediated Expression of the Tryptophan Oxygenase Gene by the SWI/SNF Complex during Liver Development

The chromatin remodeling complex, SWI/SNF, is known to regulate the transcription of several genes by altering the chromatin structure in an ATP-dependent manner. SWI/SNF exclusively contains BRG1 or BRM as an ATPase subunit. In the present study, we studied the role of SWI/SNF containing BRM or BRG...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 2005-10, Vol.138 (4), p.457-465
Main Authors: Inayoshi, Yujin, Kaneoka, Hidenori, Machida, Yuichi, Terajima, Masaomi, Dohda, Takeaki, Miyake, Katsuhide, Iijima, Shinji
Format: Article
Language:English
Subjects:
Animals
Chromatin Assembly and Disassembly
Chromosomal Proteins, Non-Histone - metabolism
Down-Regulation
Gene Expression Regulation, Developmental
Gene Silencing
glucocorticoid receptor
glucocorticoid responsive element
GRE
HDAC
histone deacetylase
Liver - growth & development
Liver - metabolism
MMTV
mouse mammary tumor virus
pRb
primary hepatocytes
Receptors, Glucocorticoid - metabolism
retinoblastoma protein
RNA, Small Interfering - genetics
siRNA
small interfering RNA
SWI/SNF
TAT
Transcription Factors - metabolism
Transcription, Genetic
transcriptional repression
tryptophan oxygenase
Tryptophan Oxygenase - genetics
Tryptophan Oxygenase - metabolism
tyrosine aminotransferase
Tyrosine Transaminase - genetics
Tyrosine Transaminase - metabolism
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Summary:The chromatin remodeling complex, SWI/SNF, is known to regulate the transcription of several genes by altering the chromatin structure in an ATP-dependent manner. SWI/SNF exclusively contains BRG1 or BRM as an ATPase subunit. In the present study, we studied the role of SWI/SNF containing BRM or BRG1 in the expression of the liver-specific tryptophan oxygenase (TO) and tyrosine aminotransferase genes. Chromatin remodeling factors significantly repressed the expression of these genes induced by glucocorticoid receptor and dexamethasone. Since the repression was not reversed by trichostatin A treatment, it seemed to be independent of the well-known histone deacetylase pathway. Knock-down of BRG1 by small interfering RNA reversed the repression in primary fetal hepatocytes. These results support a model in which SWI/SNF containing BRG1 represses late stage-specific TO gene expression at an early stage of liver development.
ISSN:0021-924X
1756-2651
DOI:10.1093/jb/mvi147