Lactobacilli binding human A-antigen expressed in intestinal mucosa

Adherent lactic acid bacteria (LAB) in the human intestine were investigated using the surface plasmon resonance technique with the biosensor BIACORE-1000. Ninety-three LAB strains were isolated from human feces and evaluated for binding to human blood type-A antigen [GalNAc α1-3 (Fuc α1-2) Gal-: A-...

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Bibliographic Details
Published in:Research in microbiology 2006-09, Vol.157 (7), p.659-665
Main Authors: Uchida, Hideaki, Kinoshita, Hideki, Kawai, Yasushi, Kitazawa, Haruki, Miura, Koh, Shiiba, Kenichi, Horii, Akira, Kimura, Katsunori, Taketomo, Naoki, Oda, Munehiro, Yajima, Takaji, Saito, Tadao
Format: Article
Language:English
Subjects:
ABO Blood-Group System - metabolism
Adherence
Bacterial Adhesion
Bacterial Proteins - metabolism
Biological and medical sciences
Biosensing Techniques
Feces - microbiology
Fundamental and applied biological sciences. Psychology
Human A-antigen
Humans
Intestinal Mucosa - metabolism
Lactic acid bacteria
Lactobacillus
Lactobacillus - classification
Lactobacillus - isolation & purification
Lactobacillus - metabolism
Microbiology
Protein Binding
Surface layer protein
Surface Plasmon Resonance
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Summary:Adherent lactic acid bacteria (LAB) in the human intestine were investigated using the surface plasmon resonance technique with the biosensor BIACORE-1000. Ninety-three LAB strains were isolated from human feces and evaluated for binding to human blood type-A antigen [GalNAc α1-3 (Fuc α1-2) Gal-: A-trisaccharide] expressed in the intestinal mucosa. Eleven strains showed strong adherence to an A-trisaccharide biotinyl polymer (BP) probe, and slightly or no adherence to a B-trisaccharide BP probe. Four strains with high adherence (high A/B ratio) were selected and their surface layer proteins (SLPs) were evaluated for A-antigen ligand binding using BIACORE. The SLP from L. brevis strain OLL2772 showed a single band at ca. 48 kDa by SDS–PAGE analysis and it had a very strong adherence to the human A-antigen, as shown using an anti-A lectin blocking technique. A partial N-terminal sequence of the band showed strong homology to an S-layer protein of L. brevis ATCC8287 T. The probiotic LAB binds to human blood type-A antigen expressed in the intestinal mucosa which may aid in colonization of the gut.
ISSN:0923-2508
1769-7123
DOI:10.1016/j.resmic.2006.03.001