Regulation of nitrate reductase by non-modifiable derivatives of PII in the cells of Synechococcus elongatus strain PCC 7942

In Synechococcus elongatus, the PII protein inhibits both transport and reduction of nitrate when ammonium is present in the medium. Using a transporter mutant having ammonium-resistant nitrate transport activity as the genetic background, we analyzed specific effects of PII on in vivo nitrate reduc...

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Bibliographic Details
Published in:Plant and cell physiology 2006-08, Vol.47 (8), p.1182-1186
Main Authors: Takatani, N.(Nagoya Univ. (Japan)), Kobayashi, M, Maeda, S, Omata, T
Format: Article
Language:English
Subjects:
CYANOBACTERIA
Gene Expression Regulation, Bacterial
METABOLISME DE L'AZOTE
METABOLISMO DEL NITROGENO
NITRATE
NITRATE REDUCTASE
Nitrate Reductase - metabolism
NITRATES
Nitrates - metabolism
NITRATO REDUCTASA
NITRATOS
NITROGEN METABOLISM
Phosphorylation
PII Nitrogen Regulatory Proteins - metabolism
PII Nitrogen Regulatory Proteins - physiology
Synechococcus - enzymology
Synechococcus elongatus
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Summary:In Synechococcus elongatus, the PII protein inhibits both transport and reduction of nitrate when ammonium is present in the medium. Using a transporter mutant having ammonium-resistant nitrate transport activity as the genetic background, we analyzed specific effects of PII on in vivo nitrate reductase activity by measuring uptake of nitrate from the medium. The results showed that the regulation of nitrate reductase does not require changes in the electric charge or size of the side chain at the phosphorylation site of PII. Phosphorylation of PII is thus unlikely to play a role in the regulation of nitrate reductase.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pcj088