Remodeling of extracellular matrix at ovulation of the bovine ovarian follicle

• Published in: Molecular reproduction and development 2006-10, Vol.73 (10), p.1292-1302
• Main Authors: Irving-Rodgers, H.F., Catanzariti, K.D., Aspden, W.J., D'Occhio, M.J., Rodgers, R.J.
• Format: Article
• Language: English
• Subjects: Animals; basal lamina; Basement Membrane - cytology; Basement Membrane - metabolism; Biological and medical sciences; Cattle; Chondroitin Sulfate Proteoglycans - analysis; Chondroitin Sulfate Proteoglycans - genetics; Chondroitin Sulfate Proteoglycans - metabolism; Collagen Type IV - analysis; Collagen Type IV - genetics; Collagen Type IV - metabolism; Estradiol - pharmacology; extracellular matrix; Extracellular Matrix - chemistry; Extracellular Matrix - metabolism; Female; focimatrix; follicle; Fundamental and applied biological sciences. Psychology; Heparan Sulfate Proteoglycans - genetics; Heparan Sulfate Proteoglycans - metabolism; Immunohistochemistry; Laminin - genetics; Laminin - metabolism; Lectins, C-Type - analysis; Lectins, C-Type - genetics; Lectins, C-Type - metabolism; Mammalian female genital system; Membrane Glycoproteins - genetics; Membrane Glycoproteins - metabolism; Morphology. Physiology; Ovarian Follicle - chemistry; Ovarian Follicle - drug effects; Ovarian Follicle - metabolism; ovulation; Ovulation - genetics; Ovulation - metabolism; Progesterone - pharmacology; Prostaglandins - pharmacology; RNA, Messenger - analysis; RNA, Messenger - metabolism; Versicans; Vertebrates: reproduction
• ISSN: 1040-452X; 1098-2795
• DOI: 10.1002/mrd.20580

Using immunohistochemistry and RNA analyses we examined the fate of components of a newly identified matrix that develops between granulosa cells (focimatrix, abbreviated from focal intraepithelial matrix) and of the follicular basal lamina in ovulating bovine ovarian follicles. Pre‐ and postovulatory follicles were generated by treatment with estradiol (Day 1), progesterone (Days 1–10), and prostaglandin analogue (Day 9) with either no further treatment (Group 1, n = 6) and or with 25 mg porcine LH (Day 11, Group 2, n = 8 or Day 10, Group 3, n = 8) and ovariectomy on Day 12 (12–14 hr post LH in Group 2, 38–40.5 hr in Group 3). In the time frame examined no loss of follicular basal lamina laminin chains β2 and γ1 or nidogen 1 was observed. In the follicular basal lamina collagen type IV α1 and perlecan were present prior to ovulation; after ovulation collagen type IV α1 was discontinuously distributed and perlecan was absent. Versican in the theca interna adjacent to the follicular basal lamina in preovulatory follicles was not observed post ovulation, however, the granulosa cells then showed strong cytoplasmic staining for versican. Expression of versican isoforms V0, V1, and V3 was detected at all stages. Focimatrix was observed in preovulatory follicles. It contained collagen type IV α1, laminins β2 and γ1, nidogen 1 and perlecan and underwent changes in composition similar to that of the follicular basal lamina. In conclusion focimatrix and the follicular basal lamina are degraded at ovulation. Individual components are lost at different times. Mol. Reprod. Dev. © 2006 Wiley‐Liss, Inc.