Proteomic analysis of Agrobacterium tumefaciens response to the vir gene inducer acetosyringone

Agrobacterium tumefaciens causes crown gall disease in a wide range of plants by transforming plants through the transfer and integration of its transferred DNA (T‐DNA) into the host genome. In the present study, we used two‐dimensional gel electrophoresis to examine the protein expression profiles...

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Published in:Proteomics (Weinheim) 2006-07, Vol.6 (14), p.4130-4136
Main Authors: Lai, Erh-Min, Shih, Hsiao-Wei, Wen, Sy-Ray, Cheng, Min-Wen, Hwang, Hau-Hsuan, Chiu, Shih-Hua
Format: Article
Language:English
Subjects:
Acetophenones - pharmacology
Acetosyringone
Agrobacterium tumefaciens
Agrobacterium tumefaciens - drug effects
Agrobacterium tumefaciens - metabolism
Analytical, structural and metabolic biochemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Miscellaneous
Plant transformation
Proteins
Proteome - analysis
Virulence
Virulence Factors - genetics
Virulence Factors - metabolism
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Summary:Agrobacterium tumefaciens causes crown gall disease in a wide range of plants by transforming plants through the transfer and integration of its transferred DNA (T‐DNA) into the host genome. In the present study, we used two‐dimensional gel electrophoresis to examine the protein expression profiles of A. tumefaciens in response to the phenolic compound acetosyringone (AS), a known plant‐released virulence (vir) gene inducer. Using mass spectrometry, we identified 11 proteins consisting of 9 known AS‐induced Vir proteins and 2 newly discovered AS‐induced proteins, an unknown protein Y4mC (Atu6162) and a small heat shock protein HspL (Atu3887). Further expression analysis revealed that the AS‐induced expression of Y4mC and HspL is regulated by the VirA/VirG two‐component system. This report presents the first proteomics study successfully identifying both known and new AS‐induced proteins that are implicated in Agrobacterium virulence.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200600254