Platelet-endothelial cell adhesion molecule-1 (CD31) recycles and induces cell growth inhibition on human tumor cell lines

CD31 (PECAM‐1) is a 130‐kDa member of the immunoglobulin gene superfamily expressed on endothelial cells, platelets, and most leukocytes. This report demonstrates by Western Blot and immunofluorescence that some human melanoma and adenocarcinoma cell lines express CD31 on the cell surface. The surfa...

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Bibliographic Details
Published in:Journal of cellular biochemistry 2006-08, Vol.98 (5), p.1334-1350
Main Authors: Lutzky, Viviana P., Carnevale, Romina P., Alvarez, Mariano J., Maffia, Paulo C., Zittermann, Sandra I., Podhajcer, Osvaldo L., Issekutz, Andrew C., Chuluyan, H. Eduardo
Format: Article
Language:English
Subjects:
adenocarcinoma
Annexin A5 - metabolism
Antibodies - immunology
apoptosis
CD31
Cell Communication
cell growth
Cell Line, Tumor
Cell Proliferation
Culture Media, Serum-Free
Humans
melanoma
Neoplasms - metabolism
Neoplasms - pathology
Oligonucleotides, Antisense - genetics
PECAM-1
Platelet Endothelial Cell Adhesion Molecule-1 - genetics
Platelet Endothelial Cell Adhesion Molecule-1 - immunology
Platelet Endothelial Cell Adhesion Molecule-1 - metabolism
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Summary:CD31 (PECAM‐1) is a 130‐kDa member of the immunoglobulin gene superfamily expressed on endothelial cells, platelets, and most leukocytes. This report demonstrates by Western Blot and immunofluorescence that some human melanoma and adenocarcinoma cell lines express CD31 on the cell surface. The surface expression of CD31 was regulated by cell–cell contact, being higher on sparse and spontaneously detached cells. Indeed, fixing and permeabilizing tumor cells revealed a cytoplasmic pool, which was confirmed by confocal microscopy. Some of the plasma surface molecule is endocytosed following mAb binding. Engagement of CD31 on tumor cells via domain‐3 inhibited proliferation by inducing cell apoptosis. On the other hand, apoptosis does not increase CD31 expression. Overall, these results indicate that there is an intracellular pool of CD31 on some tumor cells, which modulates CD31 surface expression and its role in cancer cell growth and metastasis. Thus, the expression of CD31 and its role in cell survival in some tumor cells appears to differ from endothelial cells. J. Cell. Biochem. 98: 1334–1350, 2006. © 2006 Wiley‐Liss, Inc.
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.20769