Gene analysis of Cryptosporidium parvum HNJ-1 strain isolated in Japan

We analyzed genetically Cryptosporidium parvum HNJ-1 strain, which is the Japanese reference strain isolated from human in Japan. DNA sequences of genes for thrombospondin-related adhesive protein of Cryptosporidium-1 and Cryptosporidium-2 (TRAP-C1, TRAP-C2), heat shock protein 70 (HSP70), oocyst wa...

Full description

Saved in:
Bibliographic Details
Published in:Parasitology research (1987) 2005-12, Vol.97 (6), p.452-457
Main Authors: SATOH, Masaaki, KIMATA, Isao, ISEKI, Motohiro, NAKAI, Yutaka
Format: Article
Language:English
Subjects:
Adult
Amino Acid Substitution
Animals
Biological and medical sciences
Cryptosporidiosis - parasitology
Cryptosporidium parvum
Cryptosporidium parvum - chemistry
Cryptosporidium parvum - classification
Cryptosporidium parvum - genetics
Female
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Genes, Protozoan
Humans
Invertebrates
Japan
Peptide Elongation Factor 1 - genetics
Protozoan Proteins - genetics
RNA, Protozoan - genetics
RNA, Ribosomal, 18S - genetics
Sequence Analysis, Protein
Species Specificity
Tubulin - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We analyzed genetically Cryptosporidium parvum HNJ-1 strain, which is the Japanese reference strain isolated from human in Japan. DNA sequences of genes for thrombospondin-related adhesive protein of Cryptosporidium-1 and Cryptosporidium-2 (TRAP-C1, TRAP-C2), heat shock protein 70 (HSP70), oocyst wall protein (COWP), beta-tubulin, alpha-tubulin, polythreonine-region (Poly-T), elongation factor 1 alpha (EF-1α), and 18S rRNA of this strain were determined. They showed high rate of homology to published sequences of genotype 2 strains, which were considered to be infective to both humans and animals. However, HNJ-1 had synonymous and non-synonymous substitutions in the nucleotide sequence of TRAP-C1 and beta-tubulin among HNJ-1 and published sequences of genotype 2 strains. These results implied that HNJ-1 strain was an unique subpopulation of genotype 2 strain of C. parvum.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-005-1474-8