Antiproliferative effect of diallyl disulfide (DADS) on prostate cancer cell line LNCaP

• Published in: Cell biochemistry and function 2006-09, Vol.24 (5), p.407-412
• Main Authors: Gunadharini, D. N., Arunkumar, A., Krishnamoorthy, G., Muthuvel, R., Vijayababu, M. R., Kanagaraj, P., Srinivasan, N., Aruldhas, M. M., Arunakaran, J.
• Format: Article
• Language: English
• Subjects: Acid Phosphatase; Allium sativum; Allyl Compounds - pharmacology; Androgens - metabolism; Anticarcinogenic Agents - pharmacology; apoptosis; Cell Division - drug effects; Cell Line, Tumor; diallyl disulfide; Disulfides - pharmacology; DNA fragmentation; DNA Fragmentation - drug effects; Garlic; Humans; L-Lactate Dehydrogenase - metabolism; Male; prostate cancer cells; prostate-specific antigen; Prostate-Specific Antigen - metabolism; Prostatic Neoplasms - drug therapy; Protein Tyrosine Phosphatases - metabolism
• ISSN: 0263-6484; 1099-0844
• DOI: 10.1002/cbf.1262

Garlic has been used throughout the world to treat coughs, toothache, earache, dandruff, hypertension, hysteria, diarrhoea, dysentery, diptheria, vaginitis and many other conditions. Garlic contains a complex mixture of oil and water‐soluble organosulfur compounds. Diallyl disulfide (DADS), an oil‐soluble constituent of garlic seems to be effective in reducing tumour cells originating from colon, lung and skin. Hence our present study focuses on the dose‐dependent effect of DADS on an androgen‐dependent prostate cancer cell line. Various concentrations of DADS ranging from 25 to 100 µM were given to LNCaP cells and the activity of lactate dehydrogenase (LDH) prostatic acid phosphatase (PAcP) and the level of prostate specific antigen were studied. DADS reduced the secretory activity of LNCaP cells with the gradual increase in dosage. DADS was found to act as a good antiproliferative agent, which was confirmed by proliferation assay. DADS also induced apoptosis and nuclear segmentation in the higher doses. Copyright © 2005 John Wiley & Sons, Ltd.