Endophyte Fungal Isolates from Podophyllum peltatum Produce Podophyllotoxin

The lignan podophyllotoxin (1) is highly valued as the precursor to clinically useful anticancer drugs. Substantial drug development of this compound class continues, including potential new use for inflammatory disease. We have isolated two endophyte fungi, both strains of Phialocephala fortinii, f...

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Bibliographic Details
Published in:Journal of natural products (Washington, D.C.) D.C.), 2006-08, Vol.69 (8), p.1121-1124
Main Authors: Eyberger, Amy L, Dondapati, Rajeswari, Porter, John R
Format: Article
Language:English
Subjects:
Animals
Antineoplastic Agents, Phytogenic - chemistry
Antineoplastic Agents, Phytogenic - isolation & purification
Antineoplastic Agents, Phytogenic - pharmacology
Artemia - drug effects
Ascomycota - chemistry
Ascomycota - metabolism
Biological and medical sciences
Biotechnology
DNA, Fungal - chemistry
DNA, Fungal - genetics
DNA, Fungal - isolation & purification
Fundamental and applied biological sciences. Psychology
General pharmacology
Health. Pharmaceutical industry
Industrial applications and implications. Economical aspects
Medical sciences
Molecular Structure
Other active biomolecules
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Phialocephala fortinii
Podophyllotoxin - chemistry
Podophyllotoxin - isolation & purification
Podophyllotoxin - pharmacology
Podophyllum peltatum
Podophyllum peltatum - chemistry
Prodrugs - chemistry
Prodrugs - isolation & purification
Prodrugs - pharmacology
Production of active biomolecules
Rhizome - chemistry
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Summary:The lignan podophyllotoxin (1) is highly valued as the precursor to clinically useful anticancer drugs. Substantial drug development of this compound class continues, including potential new use for inflammatory disease. We have isolated two endophyte fungi, both strains of Phialocephala fortinii, from rhizomes of the plant Podophyllum peltatum. The fungi were identified through DNA sequencing and morphology. Both strains of fungi are slow-growing and produce 1 at low but measurable amounts in broth culture. The compound was confirmed through matching HPLC retention times, absorption spectra, and MS data to authentic 1. The yield of 1 has ranged from 0.5 to 189 μg/L in 4 weeks of culture. These fungi have implications for the sustained production of 1 independent of wild populations of the source plants.
ISSN:0163-3864
1520-6025
DOI:10.1021/np060174f