Synthesis and in vitro anti-cancer evaluation of tamoxifen-loaded magnetite/PLLA composite nanoparticles

The present study deals with the synthesis and characterization of tamoxifen-loaded magnetite/poly( l-lactic acid) composite nanoparticles (TMCN), and their in vitro anti-cancer activity against MCF-7 breast cancer cells. The composite nanoparticles with an average size of ∼200 nm, were synthesized...

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Bibliographic Details
Published in:Biomaterials 2006-11, Vol.27 (33), p.5725-5733
Main Authors: Hu, F.X., Neoh, K.G., Kang, E.T.
Format: Article
Language:English
Subjects:
Antineoplastic Agents - chemistry
Antineoplastic Agents - pharmacology
Cell Line, Tumor - drug effects
Cell Shape
Composite nanoparticles
Controlled release
Drug Carriers - chemistry
Estrogen Antagonists - chemistry
Estrogen Antagonists - pharmacology
Ferrosoferric Oxide - chemistry
Ferrosoferric Oxide - pharmacology
Humans
Lactic Acid - chemistry
Lactic Acid - pharmacology
Magnetic drug targeting
MRI
Nanoparticles - chemistry
Nanoparticles - ultrastructure
Particle Size
Polyesters
Polymers - chemistry
Polymers - pharmacology
Tamoxifen
Tamoxifen - chemistry
Tamoxifen - pharmacology
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Summary:The present study deals with the synthesis and characterization of tamoxifen-loaded magnetite/poly( l-lactic acid) composite nanoparticles (TMCN), and their in vitro anti-cancer activity against MCF-7 breast cancer cells. The composite nanoparticles with an average size of ∼200 nm, were synthesized via a solvent evaporation/extraction technique in an oil/water emulsion. The superparamagnetic property (saturation magnetization value of ∼7 emu/g) of the TMCN is provided by Fe 3O 4 nanoparticles of ∼6 nm encapsulated in the poly( l-lactic acid) matrix. The encapsulation efficiency of the Fe 3O 4 and tamoxifen as a function of the concentration in the organic phase was investigated. The uptake of TMCN and tamoxifen by MCF-7 was estimated from the intracellular iron concentration. After 4 h incubation of MCF-7 with TMCN, significant changes in the cell morphology were discernible from phase contrast microscopy. Cytotoxicity assay shows that while the Fe 3O 4-loaded poly( l-lactic acid) composite nanoparticles exhibit no significant cytotoxicity against MCF-7, ∼80% of the these cells were killed after incubation for 4 days with TMCN.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2006.07.014