Depletion of vesicle-tethering factor p115 causes mini-stacked Golgi fragments with delayed protein transport

Depletion of p115 with small interfering RNA caused fragmentation of the Golgi apparatus, resulting in dispersed distribution of stacked short cisternae and a vesicular structure (mini-stacked Golgi). The mini-stacked Golgi with cis- and trans-organization is functional in protein transport and glyc...

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Published in:Biochemical and biophysical research communications 2005-12, Vol.338 (2), p.1268-1274
Main Authors: Sohda, Miwa, Misumi, Yoshio, Yoshimura, Shin-ichiro, Nakamura, Nobuhiro, Fusano, Takami, Sakisaka, Shotaro, Ogata, Shigenori, Fujimoto, Junichro, Kiyokawa, Nobutaka, Ikehara, Yukio
Format: Article
Language:English
Subjects:
Breferdin A
Drosophila Proteins - deficiency
Endoplasmic Reticulum - metabolism
Endoplasmic Reticulum - ultrastructure
ER-Golgi transport
Gene Silencing
Golgi apparatus
Golgi Apparatus - metabolism
Golgi Apparatus - ultrastructure
HeLa Cells
Humans
Membrane Proteins - deficiency
p115
Protein Transport - physiology
siRNA
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Summary:Depletion of p115 with small interfering RNA caused fragmentation of the Golgi apparatus, resulting in dispersed distribution of stacked short cisternae and a vesicular structure (mini-stacked Golgi). The mini-stacked Golgi with cis- and trans-organization is functional in protein transport and glycosylation, although secretion is considerably retarded in p115 knockdown cells. The fragmented Golgi was further disrupted by treatment with breferdin A and reassembled into the mini-stacked Golgi by removal of the drug, as observed in control cells. In addition, p115 knockdown cells maintained retrograde transport from the Golgi to the endoplasmic reticulum, although the rate was not as efficient as in control cells. While no alternation of microtubule networks was found in p115 knockdown cells, the fragmented Golgi resembled those in cells treated with anti-microtubule drugs. The results suggest that p115 is involved in vesicular transport between endoplasmic reticulum and the Golgi, along with microtubule networks.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2005.10.084