Further studies on leptin and leptin receptor expression in myometrium and uterine myomas

Examination of the potential role of leptin in the development of uterine myomas. Expression of the leptin gene and leptin receptor gene was tested in the myometrium of healthy women, and in myomas and the surrounding myometrium of women with benign tumors. Using RT-PCR, expression of the leptin gen...

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Bibliographic Details
Published in:European journal of gynaecological oncology 2005, Vol.26 (5), p.517-525
Main Authors: Markowska, A, Rucinski, M, Drews, K, Malendowicz, L K
Format: Article
Language:English
Subjects:
Adult
Aged
Case-Control Studies
DNA Primers
Female
Gene Expression Regulation, Neoplastic
Humans
Leiomyoma - metabolism
Leptin - metabolism
Middle Aged
Myometrium - metabolism
Receptors, Cell Surface - metabolism
Receptors, Leptin
Reverse Transcriptase Polymerase Chain Reaction
Uterine Neoplasms - metabolism
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Summary:Examination of the potential role of leptin in the development of uterine myomas. Expression of the leptin gene and leptin receptor gene was tested in the myometrium of healthy women, and in myomas and the surrounding myometrium of women with benign tumors. Using RT-PCR, expression of the leptin gene and leptin receptor gene were studied in myomas and in the surrounding myometrium in 30 women with uterine myomas at various phases of the menstrual cycle, and in the myometrium of ten women in a control group. Presence of leptin gene proteins and leptin receptor gene proteins in the women was also examined by Western blotting. Using RT-PCR, expression of the leptin gene was demonstrated both in myomas and in the surrounding myometrium. In contrast, expression of the gene could not be detected in the myometrium of healthy women. The results were confirmed by Western blotting, which documented the identical distribution of leptin proteins and leptin receptor proteins in studied tissues. Demonstration of the expression of leptin genes and leptin proteins in uterine myomas and in the surrounding myometrium, and their absence in the myometrium of healthy women suggests the involvement of leptin in the development of uterine myomas.
ISSN:0392-2936