Association between the Infectivity of Mycobacterium tuberculosis Strains and Their Efficiency for Extrarespiratory Infection

Extrarespiratory tuberculosis is determined mainly by impaired immunity of the host. The additional role played by bacterial factors in determining whether an infection by Mycobacterium tuberculosis disseminates to extrarespiratory sites has not been analyzed in depth. In the present study, we selec...

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Bibliographic Details
Published in:The Journal of infectious diseases 2005-12, Vol.192 (12), p.2059-2065
Main Authors: García de Viedma, Darío, Lorenzo, Gema, Cardona, Pere-Joan, Alonso Rodriguez, Noelia, Gordillo, Sergi, Ruiz Serrano, María Jesús, Bouza, Emilio
Format: Article
Language:English
Subjects:
Animals
Bacteria
Biological and medical sciences
Cells, Cultured
Coinfection
Cytokines - biosynthesis
Disease Models, Animal
Fundamental and applied biological sciences. Psychology
Humans
In Vitro Techniques
Infections
Infectious diseases
Inoculum
Lung - microbiology
Lungs
Macrophages
Macrophages - microbiology
Medical sciences
Messenger RNA
Mice
Mice, Inbred C57BL
Mice, SCID
Microbiology
Mycobacterium tuberculosis
Mycobacterium tuberculosis - pathogenicity
RNA, Messenger - analysis
Spleen
Spleen - microbiology
Survival Analysis
Tuberculosis
Tuberculosis - microbiology
Virulence
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Summary:Extrarespiratory tuberculosis is determined mainly by impaired immunity of the host. The additional role played by bacterial factors in determining whether an infection by Mycobacterium tuberculosis disseminates to extrarespiratory sites has not been analyzed in depth. In the present study, we selected patients who were dually infected with 2 M. tuberculosis strains but in whom only one of the strains infected extrarespiratory sites, whereas the other strain remained at the respiratory site. We compared the infectivity of respiratory and extrarespiratory strains in a newly designed ex vivo competitive macrophage coinfection assay and in the murine aerosol-infection model. The extrarespiratory strains infected macrophages more efficiently than did the respiratory strains, and a representative extrarespiratory strain also showed higher infectivity in vivo. Our data indicate that, in addition to host immune status, a bacterial factor—the infectivity of a M. tuberculosis strain—should be considered in determining the likelihood of extrarespiratory dissemination
ISSN:0022-1899
1537-6613
DOI:10.1086/498245