Long chain fatty acyl-CoA synthetase 5 expression is induced by insulin and glucose: involvement of sterol regulatory element-binding protein-1c

• Published in: Biochimie 2005-12, Vol.87 (12), p.1149-1155
• Main Authors: Achouri, Y., Hegarty, B.D., Allanic, D., Bécard, D., Hainault, I., Ferré, P., Foufelle, F.
• Format: Article
• Language: English
• Subjects: Animals; Coenzyme A Ligases - biosynthesis; Coenzyme A Ligases - drug effects; Coenzyme A Ligases - genetics; Coenzyme A Ligases - metabolism; Diabetes; Diabetes Mellitus, Experimental - physiopathology; Eating; Enzyme Induction; Fasting; Fatty Acids - metabolism; Female; Glucose - pharmacology; Hepatocyte; Insulin - pharmacology; Lipogenesis; Liver - enzymology; Models, Animal; Polymerase Chain Reaction; Rats; Rats, Wistar; RNA, Messenger - genetics; Sterol Regulatory Element Binding Protein 1 - physiology
• ISSN: 0300-9084; 1638-6183
• DOI: 10.1016/j.biochi.2005.04.015

In a screen for sterol regulatory element-binding protein (SREBP)-1c target genes in the liver, we identified long chain fatty acyl-CoA synthetase 5 (ACS-5). Hepatic ACS-5 mRNA is poorly expressed during fasting and diabetes and strongly induced by carbohydrate refeeding and insulin treatment. In cultured hepatocytes, insulin and a high glucose concentration induce ACS-5 mRNA. Adenoviral overexpression of a nuclear form of SREBP-1c in liver of diabetic mice or in cultured hepatocytes mimics the effect of insulin to induce ACS-5. By contrast, a dominant negative form of SREBP-1c abolishes the effect of insulin on ACS-5 expression. The dietary and SREBP-1c-mediated insulin regulation of ACS-5 expression indicate that ACS-5 is involved in the anabolic fate of fatty acids.