A novel Takeout‐like protein expressed in the taste and olfactory organs of the blowfly, Phormia regina

In insects, the functional molecules responsible for the taste system are still obscure. The gene for a 28.5 kDa protein purified from taste sensilla of the blowfly Phormia regina belongs to a gene family that includes takeout of Drosophila melanogaster. Molecular phylogenetic analysis revealed that...

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Bibliographic Details
Published in:The FEBS journal 2006-09, Vol.273 (18), p.4311-4321
Main Authors: Fujikawa, Kazuyo, Seno, Keiji, Ozaki, Mamiko
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biochemistry
Blotting, Western
Calliphoridae
chemoreceptor
Diptera - chemistry
Diptera - genetics
Drosophila melanogaster
Drosophila Proteins - chemistry
Drosophila Proteins - genetics
Electrophoresis, Gel, Two-Dimensional
fly
Gene expression
Gene Expression Regulation
Insect Proteins - chemistry
Insect Proteins - genetics
Insect Proteins - isolation & purification
Insects
Microscopy, Fluorescence
Microscopy, Immunoelectron
Molecular Sequence Data
olfaction
Phormia regina
Phylogeny
Proteins
Smell
Smell - genetics
takeout
Taste
Taste - genetics
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Summary:In insects, the functional molecules responsible for the taste system are still obscure. The gene for a 28.5 kDa protein purified from taste sensilla of the blowfly Phormia regina belongs to a gene family that includes takeout of Drosophila melanogaster. Molecular phylogenetic analysis revealed that the Phormia Takeout‐like protein is most similar to the protein encoded by a member of the Drosophila takeout gene family, CG14661, whose expression and function have not been identified yet. Western blot analyses revealed that Phormia Takeout‐like protein was exclusively expressed in antennae and labellum of the adult blowfly in both sexes. Immunohistochemical experiments demonstrated that Takeout‐like protein was localized around the lamella structure of the auxiliary cells and in the sensillar lymph of the labellar taste sensillum. In antennae, Takeout‐like protein was distributed at the base of the olfactory sensilla as well. No significant differences in Takeout‐like protein expression were found between the sexes. Our results suggest that Phormia Takeout‐like protein is involved in some early events concerned with chemoreception in both the taste and olfactory systems.
ISSN:1742-464X
1742-4658
DOI:10.1111/j.1742-4658.2006.05422.x