Proteomic Analysis of Effect of Hyperthermia on Spermatogenesis in Adult Male Mice

We characterized cellular and molecular mechanisms involved in spermatogenesis following short-term heat exposure of murine testis. For these studies, we utilized a proteomic approach with two-dimensional gel electrophoresis (2DE) analyses and mass spectroscopic identification of proteins with alter...

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Bibliographic Details
Published in:Journal of proteome research 2006-09, Vol.5 (9), p.2217-2225
Main Authors: Zhu, Ye-Fei, Cui, Yu-Gui, Guo, Xue-Jiang, Wang, Lei, Bi, Ye, Hu, Yan-Qiu, Zhao, Xin, Liu, Quan, Huo, Ran, Lin, Min, Zhou, Zuo-Min, Sha, Jia-Hao
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
Computational Biology
Electrophoresis, Gel, Two-Dimensional
Hyperthermia, Induced - adverse effects
Immunohistochemistry
Male
Mass Spectrometry
Mice
Proteins - analysis
Proteomics - methods
Spermatogenesis - physiology
Testis - cytology
Testis - physiology
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Summary:We characterized cellular and molecular mechanisms involved in spermatogenesis following short-term heat exposure of murine testis. For these studies, we utilized a proteomic approach with two-dimensional gel electrophoresis (2DE) analyses and mass spectroscopic identification of proteins with altered expression in mouse testes at different times after heat shock. We established a proteome reference map from 7-wk-old mouse testis linked to a federated proteome database. We used these tools to analyze quantitative variations in the tissue over a time course of 0.5, 2, 6, and 12 h following heat exposure. We separated 108 protein spots expressed differentially between the heat shock tissues and the control mouse testes. Of these spots, we identified 36 by comparing with the control reference map. We then focused on the heterogeneous nuclear ribonucleoproteins (hnRNPs) and the chaperonins containing t-complex polypeptide-1 (CCT). Further analysis in this heat-shocked model suggests numerous potential mechanisms for heat shock-induced spermatogenic disorder. Keywords: proteomics • mouse testis • heat shock
ISSN:1535-3893
1535-3907
DOI:10.1021/pr0600733