Expression of the adrenomedullin gene in adipose tissue

Adrenomedullin (AM) is a potent vasodilating peptide originally isolated from human pheochromocytoma cells. This report concerns the expression and secretion of AM from adipose tissue. Northern blot analysis demonstrated marked expression of AM mRNA in mouse adipose tissue. Expression levels in adip...

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Bibliographic Details
Published in:Regulatory peptides 2005-12, Vol.132 (1), p.17-22
Main Authors: Nambu, Takuo, Arai, Hiroshi, Komatsu, Yasato, Yasoda, Akihiro, Moriyama, Kenji, Kanamoto, Naotetsu, Itoh, Hiroshi, Nakao, Kazuwa
Format: Article
Language:English
Subjects:
Adiopocyte
Adipocytes - metabolism
Adipose Tissue - metabolism
Adrenomedullin
Animals
Body Mass Index
Fat
Gene Expression
Humans
Male
Mice
Mice, Inbred C57BL
Mice, Obese
ob/ob mice
Obesity
Obesity - metabolism
Peptides - genetics
Peptides - metabolism
Protein Biosynthesis
RNA, Messenger - metabolism
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Summary:Adrenomedullin (AM) is a potent vasodilating peptide originally isolated from human pheochromocytoma cells. This report concerns the expression and secretion of AM from adipose tissue. Northern blot analysis demonstrated marked expression of AM mRNA in mouse adipose tissue. Expression levels in adipose tissues were 2.5–3.2 times higher than in the kidney. AM mRNA level in mature adipocytes was 7.3 times higher than in the stroma–vascular fraction of adipose tissue. In mature adipocyte culture, time-dependent increase of AM peptide concentration in the culture medium was detected. AM expression was also detected in human subcutaneous adipose tissue. Adipose AM expression significantly increased in obesity mouse model, high-fat diet fed mice and ob/ob mice. These results suggest that adipose tissue, especially mature adipocytes, is major source of AM in the body, and that adipocyte-derived AM plays a pathophysiological role in obesity.
ISSN:0167-0115
1873-1686
DOI:10.1016/j.regpep.2005.07.006