Effect of water deprivation on aquaporin 4 (AQP4) mRNA expression in chickens ( Gallus domesticus)

Aquaporin (AQP) 4 is a member of the AQP gene family of water-selective transport proteins. We studied the effect of water deprivation on AQP4 gene expression in chickens. The nucleotide sequence of a chicken aquaporin 4 (AQP4) cDNA that encodes a protein of 335 amino acids showed high homology to m...

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Bibliographic Details
Published in:Brain research. Molecular brain research. 2005-11, Vol.141 (2), p.193-197
Main Authors: Saito, Noboru, Ikegami, Hidehiro, Shimada, Kiyoshi
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Aquaporin 4 (AQP4)
Aquaporin 4 - biosynthesis
Aquaporin 4 - genetics
Aquaporin 4 - physiology
Base Sequence
Biological and medical sciences
cDNA cloning
Chicken
Chickens - genetics
Chickens - metabolism
Coturnix - genetics
Dehydration - genetics
Dehydration - physiopathology
Fundamental and applied biological sciences. Psychology
Gallus
Gene expression
Humans
Hypothalamus - metabolism
Male
Molecular Sequence Data
Osmolar Concentration
Polymerase Chain Reaction
Rats
Real-time PCR
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Sequence Alignment
Sequence Homology, Amino Acid
Species Specificity
Vertebrates: nervous system and sense organs
Water Deprivation
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Summary:Aquaporin (AQP) 4 is a member of the AQP gene family of water-selective transport proteins. We studied the effect of water deprivation on AQP4 gene expression in chickens. The nucleotide sequence of a chicken aquaporin 4 (AQP4) cDNA that encodes a protein of 335 amino acids showed high homology to mammalian AQP4. Using Northern blotting analysis, AQP4 mRNA in chickens was observed as a band of approximately 5.5 kb in several tissues in addition to the hypothalamus, proventriculus, kidney, and breast muscle. Quantitative analysis by real-time RT-PCR analysis showed that the mRNA expression of AQP4 in the hypothalamus significantly increased after dehydration. On the other hand, the mRNA expression of AQP4 in the kidney significantly decreased after dehydration. This suggests that AQP4 may play a pivotal role in osmoregulation in the chicken brain.
ISSN:0169-328X
1872-6941
DOI:10.1016/j.molbrainres.2005.09.005