Characteristics of Flo11-dependent flocculation in Saccharomyces cerevisiae

The FLO11-encoded flocculin is required for a variety of important phenotypes in Saccharomyces cerevisiae, including flocculation, adhesion to agar and plastic, invasive growth, pseudohyphae formation and biofilm development. We present evidence that Flo11p belongs to the Flo1-type class of flocculi...

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Bibliographic Details
Published in:FEMS yeast research 2005-12, Vol.5 (12), p.1151-1156
Main Authors: Bayly, Jennifer C., Douglas, Lois M., Pretorius, Isak S., Bauer, Florian F., Dranginis, Anne M.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Binding sites
Biofilm
Biofilms
Biofilms - growth & development
Calcium
Calcium - pharmacology
Carbohydrates
Cell Adhesion
Conserved Sequence
FLO11
Flocculation
Flocculin
Glucose - pharmacology
Hydrogen-Ion Concentration
Maltose
Maltose - pharmacology
Mannose
Mannose - pharmacology
Membrane Glycoproteins
Membrane Proteins - genetics
Membrane Proteins - metabolism
MUC1
Phenotypes
Pseudohyphae
Saccharomyces cerevisiae
Saccharomyces cerevisiae - physiology
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Sucrose
Sucrose - pharmacology
Yeast
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Summary:The FLO11-encoded flocculin is required for a variety of important phenotypes in Saccharomyces cerevisiae, including flocculation, adhesion to agar and plastic, invasive growth, pseudohyphae formation and biofilm development. We present evidence that Flo11p belongs to the Flo1-type class of flocculins rather than to the NewFlo class. Both Flo1-type and NewFlo yeast flocculation are inhibited by mannose. NewFlo flocculation, however, is also inhibited by several other carbohydrates including glucose, maltose and sucrose. These differences have in at least one case been shown to reflect differences in the structure of the carbohydrate-binding site of the flocculins. We report that Flo11p-dependent flocculation is inhibited by mannose, but not by glucose, maltose or sucrose. Furthermore, Flo11p contains a peptide sequence highly similar to one that has been shown to characterise Flo1-type flocculins. Further characterisation of the properties of Flo11p-dependent flocculation revealed that it is dependent on calcium, occurs only at cell densities greater than 1 × 10 8 ml −1, and only occurs at acidic pH.
ISSN:1567-1356
1567-1364
DOI:10.1016/j.femsyr.2005.05.004