Genetic variability of Blastocystis hominis isolates in China

To determine if genetic diversity of Blastocystis hominis exists in China, 35 B. hominis isolates obtained from 19 asymptomatic infected individuals and 16 patients with gastrointestinal symptoms were genotyped by PCR using seven pairs of known sequenced-tagged site (STS) primers. Out of the 35 isol...

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Bibliographic Details
Published in:Parasitology research (1987) 2006-10, Vol.99 (5), p.597-601
Main Authors: Yan, Yiming, Su, Shuilian, Lai, Riyong, Liao, Hua, Ye, Jinhua, Li, Xiaobo, Luo, Xiaoting, Chen, Guifeng
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blastocystis hominis
Blastocystis hominis - genetics
Blastocystis hominis - isolation & purification
Blastocystis hominis - pathogenicity
Blastocystis Infections - parasitology
China
DNA, Ribosomal - genetics
Feces - parasitology
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Genetic Variation
Genotype
Invertebrates
Phenotype
Polymerase Chain Reaction
Sequence Tagged Sites
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Summary:To determine if genetic diversity of Blastocystis hominis exists in China, 35 B. hominis isolates obtained from 19 asymptomatic infected individuals and 16 patients with gastrointestinal symptoms were genotyped by PCR using seven pairs of known sequenced-tagged site (STS) primers. Out of the 35 isolates, 29 were identified as one of the known genotypes, while five isolates showed two distinct genotypes, and one isolate was an unknown genotype as this was negative with all the STS primers. In this study, none of the isolates was classified as subtypes 4-7. Compared with the spectrum of human B. hominis subtypes obtained from five geographically different countries (Japan, Pakistan, Bangladesh, Germany, and Thailand), these results showed that subtype 1 was more a popular genotype (18/35) in China. In addition, two groups of the isolates from 19 asymptomatic infected individuals and those from 16 patients with intestinal symptoms were compared with the PCR-based subtype classification. The results suggest a possible relationship between subtype 1 and a pathogenic potential of this parasite.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-006-0186-z