An improved method for Southern DNA and Northern RNA blotting using a Mupid®-2 Mini-Gel electrophoresis unit

An improved method for Southern DNA and Northern RNA blotting using the Mupid®-2 Mini-Gel System is described. We get sharp and clear bands in Southern and Northern blotting after only 30 min short gel electrophoresis instead of the several hours large gel electrophoresis of conventional methods. Th...

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Bibliographic Details
Published in:Journal of biochemical and biophysical methods 2006-08, Vol.68 (2), p.139-143
Main Authors: Furuya, Hirokazu, Yamada, Takeshi, Ikezoe, Koji, Ohyagi, Yasumasa, Fukumaki, Yasuyuki, Fujii, Naoki
Format: Article
Language:English
Subjects:
Blotting, Northern - methods
Blotting, Southern - methods
DNA - analysis
Electrophoresis, Agar Gel - methods
Formaldehyde
Humans
Mupid®-2 system
Northern blotting
Nucleic Acid Hybridization - methods
Pulse-like electrical current
RNA - analysis
Southern blotting
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Summary:An improved method for Southern DNA and Northern RNA blotting using the Mupid®-2 Mini-Gel System is described. We get sharp and clear bands in Southern and Northern blotting after only 30 min short gel electrophoresis instead of the several hours large gel electrophoresis of conventional methods. The high electrical voltage with a pulse-like current of the Mupid®-2 Mini-Gel System also allows reduction of the amount of formaldehyde, a harmful reagent, from the gel running buffer in RNA blotting. This minor modification of DNA and RNA blotting technique enables us to perform the complete experimental procedure more quickly economically in less space, than conventional Southern and Northern blotting, as well as using an extremely small amount of formaldehyde in RNA blotting.
ISSN:0165-022X
1872-857X
DOI:10.1016/j.jbbm.2006.04.005