Chromosome Rearrangements in Canine Fibrosarcomas

We have previously reported the use of six- and seven-color paint sets in the analysis of canine soft tissue sarcomas. Here we combine this technique with flow sorting of translocation chromosomes, reverse painting, and polymerase chain reaction (PCR) analysis of the gene content of the reverse pain...

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Bibliographic Details
Published in:The Journal of heredity 2005-11, Vol.96 (7), p.766-773
Main Authors: Sargan, D. R, Milne, B. S, Aguirre Hernandez, J, O'Brien, P. C. M, Ferguson-Smith, M. A, Hoather, T, Dobson, J. M
Format: Article
Language:English
Subjects:
Activin Receptors, Type I - genetics
Animals
Cancer
chromosome aberrations
Chromosome Breakage - genetics
Chromosome Painting - methods
Chromosome Painting - veterinary
chromosome rearrangements
chromosome translocation
Chromosomes
Chromosomes, Mammalian - genetics
cytogenetics
DNA Primers - genetics
dog diseases
Dog Diseases - genetics
Dogs
Female
fibrosarcoma
Fibrosarcoma - genetics
Fibrosarcoma - veterinary
fluorescence in situ hybridization
In Situ Hybridization, Fluorescence - veterinary
Karyotyping - veterinary
Metaphase - genetics
polymerase chain reaction
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - veterinary
Protein-Serine-Threonine Kinases
Receptors, Transforming Growth Factor beta - genetics
Soft Tissue Neoplasms - genetics
Soft Tissue Neoplasms - veterinary
Translocation, Genetic - genetics
Tumors
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Summary:We have previously reported the use of six- and seven-color paint sets in the analysis of canine soft tissue sarcomas. Here we combine this technique with flow sorting of translocation chromosomes, reverse painting, and polymerase chain reaction (PCR) analysis of the gene content of the reverse paint in order to provide a more detailed analysis of cytogenetic abnormalities in canine tumors. We examine two fibrosarcomas, both from female Labrador retrievers, and show abnormalities in chromosomes 11 and 30 in both cases. Evidence of involvement of TGFBR1 is presented for one tumor.
ISSN:0022-1503
1465-7333
DOI:10.1093/jhered/esi122