Association of Matrix Metalloproteinase‐9 Promoter Gene Polymorphism With Chronic Periodontitis

• Published in: Journal of periodontology (1970) 2006-09, Vol.77 (9), p.1510-1514
• Main Authors: Keles, Gonca Cayir, Gunes, Sezgin, Sumer, A. Pinar, Sumer, Mahmut, Kara, Nurten, Bagci, Hasan, Koprulu, Hulya
• Format: Article
• Language: English
• Subjects: Adult; Aged; Case-Control Studies; Chi-Square Distribution; Chronic Disease; Chronic periodontitis; Dentistry; Female; gelatinase; Gene Frequency; Humans; Male; Matrix Metalloproteinase 9 - genetics; matrix metalloproteinase‐9; Middle Aged; Periodontal Attachment Loss - enzymology; Periodontal Attachment Loss - genetics; Periodontitis - enzymology; Periodontitis - genetics; Polymerase Chain Reaction; polymorphism; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide; Promoter Regions, Genetic
• ISSN: 0022-3492; 1943-3670
• DOI: 10.1902/jop.2006.050378

Background: Matrix metalloproteinases (MMPs) are related to tissue destruction and remodeling events in periodontal diseases. A single nucleotide polymorphism in the promoter region of human MMP‐9 gene is associated with the risk of some inflammatory diseases. Therefore, the aim of this study was to investigate the association between MMP‐9 promoter polymorphism and severe generalized chronic periodontitis in a Turkish population. Methods: Samples of venous blood and DNA were obtained from 70 severe generalized chronic periodontitis patients and 70 healthy subjects. The alleles of the C/T polymorphism at position −1562 in the promoter region of the MMP‐9 gene were distinguished by cutting with the SphI restriction enzyme. Genotype and allele frequencies were calculated, and data were analyzed by the χ2 test. Results: There was a significant difference in MMP‐9 genotypes between chronic periodontitis patients and healthy controls. The odds ratios for the CT genotype and the combination of CT and TT genotypes were 0.4 (95% confidence interval, 0.17 to 0.93; P = 0.02) and 0.37 (95% confidence interval, 016 to 0.85; P = 0.01) relative to the subjects with the CC genotype, respectively. Conclusion: MMP‐9 promoter gene polymorphism seems to be associated with severe generalized chronic periodontitis.