Phospho-Regulation of the Cdc14/Clp1 Phosphatase Delays Late Mitotic Events in S. pombe

In eukaryotes, exit from mitosis occurs through the inactivation of the Cdk1-cyclin B kinase complex and the reversal of its phosphorylation events. These late mitotic events are tightly regulated to occur only after the onset of anaphase and prior to cytokinesis. Central to this regulation is the c...

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Bibliographic Details
Published in:Developmental cell 2006-09, Vol.11 (3), p.423-430
Main Authors: Wolfe, Benjamin A., McDonald, W. Hayes, Yates, John R., Gould, Kathleen L.
Format: Article
Language:English
Subjects:
Biological and medical sciences
CDC2 Protein Kinase - metabolism
Cell Cycle
Cell Cycle Proteins - metabolism
Cell differentiation, maturation, development, hematopoiesis
Cell physiology
CELLCYCLE
Chromosome Segregation
Enzyme Activation
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Fungal
Mitosis
Models, Biological
Molecular and cellular biology
Phosphoprotein Phosphatases - metabolism
Phosphorylation
Protein Tyrosine Phosphatases - metabolism
Schizosaccharomyces - enzymology
Schizosaccharomyces - genetics
Schizosaccharomyces - metabolism
Schizosaccharomyces pombe
Schizosaccharomyces pombe Proteins - metabolism
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Summary:In eukaryotes, exit from mitosis occurs through the inactivation of the Cdk1-cyclin B kinase complex and the reversal of its phosphorylation events. These late mitotic events are tightly regulated to occur only after the onset of anaphase and prior to cytokinesis. Central to this regulation is the conserved Cdc14 family of protein phosphatases, whose activity reverses Cdk-dependent phosphorylation events. S. cerevisiae Cdc14 activity is restrained from dephosphorylating Cdk substrates and inactivating Cdk1 through its nucleolar sequestration prior to anaphase. Here, we describe a unique mode of Cdc14 regulation that operates prior to anaphase in fission yeast. Cdk1 phosphorylates and inhibits the catalytic activity of the Cdc14 family member, Clp1/Flp1. As Cdk1 activity declines during anaphase progression, Clp1/Flp1 autocatalytically reverses these phosphorylation events to stimulate its own activity. These findings point to a simple regulatory circuit that couples Cdk1 activation with its inactivation mediated through phosphorylation-dependent regulation of Clp1/Flp1 phosphatase activity.
ISSN:1534-5807
1878-1551
DOI:10.1016/j.devcel.2006.07.016