Optical nanoinjection of macromolecules into vital cells

A tuneable near infrared 90 MHz mode locked femtosecond laser was applied for targeted multiphoton optoporation of vital cells. Here, we demonstrate the laser assisted transfer of large amounts of exogenous materials and even macromolecules into living cells via a transient opening of the membrane a...

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Bibliographic Details
Published in:Journal of photochemistry and photobiology. B, Biology Biology, 2005-12, Vol.81 (3), p.136-142
Main Authors: Stracke, Frank, Rieman, Iris, König, Karsten
Format: Article
Language:English
Subjects:
Animals
Cell Line, Tumor
CHO Cells
Cricetinae
Female
Fluorescein-5-isothiocyanate - administration & dosage
Fluorescein-5-isothiocyanate - metabolism
Humans
Infrared Rays
Lasers - classification
Macromolecular Substances - administration & dosage
Macromolecular Substances - metabolism
Macromolecular Substances - pharmacology
Mice
Microscopy, Fluorescence
Multiphoton
Nanoinjection
Optoporation
Rats
Spectral imaging
Spectrometry, Fluorescence
Transfection
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Summary:A tuneable near infrared 90 MHz mode locked femtosecond laser was applied for targeted multiphoton optoporation of vital cells. Here, we demonstrate the laser assisted transfer of large amounts of exogenous materials and even macromolecules into living cells via a transient opening of the membrane and quantify the influx. The use of near infrared lasers also allow the optoporation of cells deep in three dimensional biological structures without photodestructive collateral effects.
ISSN:1011-1344
1873-2682
DOI:10.1016/j.jphotobiol.2005.07.006