Cloning of stanniocalcin (STC) cDNAs of divergent teleost species: Monomeric STC supports monophyly of the ancient teleosts, the osteoglossomorphs

• Published in: General and comparative endocrinology 2006-10, Vol.149 (1), p.100-107
• Main Authors: Amemiya, Yutaka, Irwin, David M., Youson, John H.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Catostomus commersoni; cDNA cloning; DNA, Complementary - genetics; Evolution, Molecular; Fishes - classification; Fishes - genetics; Glycoproteins - chemistry; Glycoproteins - genetics; Gnathonemus petersii; Homodimer; Molecular Sequence Data; Monomer; Osteoglossiforme monophyly; Phylogeny; Species Specificity; Stanniocalcin; Teleost; Teleostei
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/j.ygcen.2006.03.015

Molecular cloning of teleost stanniocalcin (STC) cDNAs was undertaken in two species of order Osteoglossiformes of subdivision Osteoglossomorpha and one species of each of orders Cypriniformes and Perciformes within the subdivision Euteleostei. The elephantnose ( Gnathonemus petersii) and the butterflyfish ( Pantadon buchholzi) are basal teleosts in different osteoglossiforme suborders yet their 218 amino acid (aa) mature hormones, from prehormones of 249 and 251 aa, respectively, have only 10 cysteine residues. A substitution for cysteine at the intermonomeric disulfide linkage site, implies that their STCs exist as monomeric peptides, as is the case with STC from another osteoglossormorph, arawana [Amemiya, Y., Marra, L.E., Reyhani, N., Youson, J.H., 2002. Stanniocalcin from an ancient teleost: a monomeric form of the hormone and a possible extracorpuscular distribution. Mol. Cell. Endocrinol. 188, 141–150]. The STC cDNA of the generalized teleost and cyprinid, the white sucker ( Catostomus commersoni), encodes a prehormone of 249 aa with a signal peptide of 31 aa and a mature protein of 218 aa that possesses 11 cysteine residues. The latter feature is consistent with a previous analysis that white sucker mature STC is a glycosylated, homodimeric peptide [Amemiya, Y., Marra, L.E., Reyhani, N., Youson, J.H., 2002. Stanniocalcin from an ancient teleost: a monomeric form of the hormone and a possible extracorpuscular distribution. Mol. Cell. Endocrinol. 188, 141–150]. An open reading frame of the STC cDNA of the derived teleost and perciforme, the smallmouth bass ( Micropterus dolomieui), encodes a prehormone of 255 aa with a signal peptide of 33 aa and a mature protein of 222 aa. The position of the 11 cysteines in smallmouth bass STC suggests that it exists as a homodimeric peptide. A phylogenetic analysis, using the new STC-1 amino acid sequences and those in the gene data base provided strong support for monophyly of the Osteoglossomorpha and indicated, with positioning of white sucker and smallmouth bass, that this molecule has some utility as a taxonomic marker. This analysis also suggested that two STC-1 gene sequences exist in multiple fish genomes, and that they may be a product of the fish-specific genome duplication. The mutation in the osteoglossomorph STC likely occurred after the appearance of the first teleosts and before movement of the tectonic plates.