Structural and molecular characterization of Shigella boydii type 16 O antigen

Shigella is a well-known human pathogen causing dysentery and their typing is solely based on the O antigens. We investigated the chemical structure and gene cluster of Shigella boydii type 16 O antigen. As judged by sugar and methylation analyses along with NMR spectroscopy data, the O antigen has...

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Bibliographic Details
Published in:Gene 2006-09, Vol.380 (1), p.46-53
Main Authors: Liu, Bin, Senchenkova, Sof'ya N., Feng, Lu, Perepelov, Andrei V., Xu, Tianzheng, Shevelev, Sergei D., Zhu, Yajuan, Shashkov, Alexander S., Zou, Ming, Knirel, Yuriy A., Wang, Lei
Format: Article
Language:English
Subjects:
Base Sequence
Carbohydrate Sequence
DNA Primers - genetics
DNA, Bacterial - genetics
Escherichia coli
Genes, Bacterial
Humans
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Multigene Family
O antigen gene cluster
O antigen polymerase gene ( wzy)
O Antigens - chemistry
O Antigens - classification
O Antigens - genetics
O Antigens - metabolism
Oligosaccharides - chemistry
Oligosaccharides - genetics
Polymerase Chain Reaction
S. boydii type 16
Shigella boydii
Shigella boydii - genetics
Shigella boydii - immunology
Shigella boydii - metabolism
Shigella boydii - pathogenicity
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Summary:Shigella is a well-known human pathogen causing dysentery and their typing is solely based on the O antigens. We investigated the chemical structure and gene cluster of Shigella boydii type 16 O antigen. As judged by sugar and methylation analyses along with NMR spectroscopy data, the O antigen has an O-acetylated branched pentasaccharide repeating O unit, which consists of two d-mannose residues ( d-Man), one residue each of d-glucuronic acid ( d-GlcA), N-acetylglucosamine ( d-GlcNAc) and d-galactose ( d-Gal), and the structure of the O unit was established. The O antigen gene cluster of S. boydii type 16 was identified and shown to contain putative genes for the synthesis of GDP- d-Man, genes encoding sugar transferases, O unit flippase (Wzx) and O antigen polymerase (Wzy) as expected. The function of the wzy gene was characterized by mutation test. Genes specific to S. boydii type 16 O antigen gene cluster were identified by screening 186 Escherichia coli and Shigella type strains, and can be used to develop PCR assays for detection of type 16 strains.
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2006.05.024