Studies of monomeric and homodimeric oxazolo[4,5- b]pyridinium cyanine dyes as fluorescent probes for nucleic acids visualization

The series of recently synthesized monomeric and homodimeric cyanine dyes based on monomethine cyanine chromophore with oxazolo[4,5- b]pyridinium and quinoline end groups [Vassilev A, Deligeorgiev T, Gadjev N, Drexhage K-H. Synthesis of novel monomeric and homodimeric cyanine dyes based on oxazolo[4...

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Bibliographic Details
Published in:Journal of biochemical and biophysical methods 2006-10, Vol.68 (3), p.155-165
Main Authors: Kovalska, V.B., Tokar, V.P., Losytskyy, M.Yu, Deligeorgiev, T., Vassilev, A., Gadjev, N., Drexhage, K.-H., Yarmoluk, S.M.
Format: Article
Language:English
Subjects:
Carbocyanines - chemistry
Dimerization
DNA - analysis
DNA - chemistry
Electrophoresis, Agar Gel
Fluorescent detection
Fluorescent Dyes - chemistry
Fluorescent probes
Gel electrophoresis
Indoles - chemistry
Monomethine cyanine dyes
Nucleic acids
Nucleic Acids - analysis
Nucleic Acids - chemistry
Oxazoles - chemistry
Pyridinium Compounds - chemistry
Spectrometry, Fluorescence
Staining and Labeling
Stains
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Summary:The series of recently synthesized monomeric and homodimeric cyanine dyes based on monomethine cyanine chromophore with oxazolo[4,5- b]pyridinium and quinoline end groups [Vassilev A, Deligeorgiev T, Gadjev N, Drexhage K-H. Synthesis of novel monomeric and homodimeric cyanine dyes based on oxazolo[4,5-b]pyridinium and quinolinium end groups for nucleic acid detection, Dyes Pigm 2005;66:135–142] were studied as possible fluorescent probes for nucleic acids detection. Significant fluorescence enhancement and intensity level (quantum yield up to 0.75) was observed for all the dyes in the presence of DNA. The oxazolo[4,5- b]pyridinium cyanines demonstrated high sensitivity as fluorescent stains for post-electrophoretic visualization of nucleic acids in agarose gels upon both VIS and UV transillumination, and the visualized band contained 0.8 ng of dsDNA.
ISSN:0165-022X
1872-857X
DOI:10.1016/j.jbbm.2006.04.006