Inhibitory effects of tilianin on the expression of inducible nitric oxide synthase in low density lipoprotein receptor deficiency mice

We investigated the effect of tilianin upon inducible nitric oxide synthesis in the plasma of low-density lipoprotein receptor knock-out (Ldlr-/-) mice fed with high cholesterol diet and in primary peritoneal macrophages of Ldlr-/- mice. High cholesterol diet induced nitric oxide production in the p...

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Bibliographic Details
Published in:Experimental & molecular medicine 2006-08, Vol.38 (4), p.445-452
Main Authors: Nam, Ki-Hoan, Choi, Jae-Hoon, Seo, Yun-Jeong, Lee, Young-Mi, Won, Yong-Sung, Lee, Mi-Ran, Lee, Mi-Ni, Park, Jong-Gil, Kim, Young-Myeong, Kim, Hyoung-Chin, Lee, Chul-Ho, Lee, Hyeong-Kyu, Oh, Sei-Ryang, Oh, Goo Taeg
Format: Article
Language:English
Subjects:
Animals
Atherosclerosis - metabolism
Down-Regulation - drug effects
Flavonoids - pharmacology
Glycosides - pharmacology
Inflammation - metabolism
Male
Mice
Mice, Knockout
NF-kappa B - metabolism
Nitric Oxide - biosynthesis
Nitric Oxide - blood
Nitric Oxide Synthase Type II - metabolism
Promoter Regions, Genetic - drug effects
Receptors, LDL - genetics
Sinus of Valsalva - metabolism
Sinus of Valsalva - pathology
Sinus of Valsalva - ultrastructure
Tissue Distribution
Tyrosine - analogs & derivatives
Tyrosine - metabolism
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Summary:We investigated the effect of tilianin upon inducible nitric oxide synthesis in the plasma of low-density lipoprotein receptor knock-out (Ldlr-/-) mice fed with high cholesterol diet and in primary peritoneal macrophages of Ldlr-/- mice. High cholesterol diet induced nitric oxide production in the plasma of Ldlr-/- mice. Tilianin reduced the level of nitric oxide (NO) in plasma from Ldlr-/- mice induced by the high cholesterol diet. Tilianin also inhibited the NO production from the primary culture of peritoneal macrophages treated with lipopolysaccharide. The inhibition of NO production was caused by the suppression of inducible nitric oxide synthase (iNOS) gene expression in peritoneal macrophages isolated from Ldlr-/- mice. Moreover, tilianin inhibited the transcriptional activation of iNOS promoter that has NF-kappaB binding element. Thus, these results provide the first evidence that tilianin inhibit iNOS expression and production of NO and may act as a potential anti-inflammatory agent.
ISSN:1226-3613
2092-6413
2092-6413
DOI:10.1038/emm.2006.52