Global Gene Expression Profiling: A Complement to Conventional Histopathologic Analysis of Neoplasia

Transcriptional profiling of entire tumors has yielded considerable insight into the molecular mechanisms of heterogeneous cell populations within different types of neoplasms. The data thus acquired can be further refined by microdissection methods that enable the analyses of subpopulations of neop...

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Bibliographic Details
Published in:Veterinary Pathology 2005-11, Vol.42 (6), p.735-752
Main Authors: Nambiar, P. R, Boutin, S. R, Raja, R, Rosenberg, D. W
Format: Article
Language:English
Subjects:
Animals
Gene Expression Profiling - methods
Gene Expression Profiling - veterinary
histopathology
Image Processing, Computer-Assisted - methods
Lasers
literature reviews
Microarray Analysis - methods
Microarray Analysis - veterinary
microarray technology
Microdissection - methods
Microdissection - veterinary
neoplasms
Neoplasms - genetics
Neoplasms - metabolism
Neoplasms - veterinary
Species Specificity
Veterinary Medicine - methods
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Summary:Transcriptional profiling of entire tumors has yielded considerable insight into the molecular mechanisms of heterogeneous cell populations within different types of neoplasms. The data thus acquired can be further refined by microdissection methods that enable the analyses of subpopulations of neoplastic cells. Separation of the various components of a neoplasm (i.e., stromal cells, inflammatory infiltrates, and blood vessels) has been problematic, primarily because of a paucity of tools for accurate microdissection. The advent of laser capture microdissection combined with powerful tools of linear amplification of RNA and high-throughput microarray-based assays have allowed the transcriptional mapping of intricate and highly complex networks within pure populations of neoplastic cells. With this approach, specific "molecular signatures" can be assigned to tumors of distinct or even similar histomorphology, thereby aiding the desired objective of pattern recognition, tumor classification, and prognostication. This review highlights the potential benefits of global gene expression profiling of tumor cells as a complement to conventional histopathologic analyses.
ISSN:0300-9858
1544-2217
DOI:10.1354/vp.42-6-735