Expression of the autofluorescent protein, DsRed2, in the recombinants of the ectomycorrhizal basidiomycete, Suillus grevillei, generated by Agrobacterium-mediated transformation

Recombinants were generated from the ectomycorrhizal basidiomycete, Suillus grevillei, through agroinfection using a binary vector carrying the hygromycin B resistance and the autofluorescent protein, DsRed2, markers. DsRed2 was driven by a cis-regulatory region of the glyceraldeyde-3-phosphate dehy...

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Bibliographic Details
Published in:Mycorrhiza 2006-09, Vol.16 (6), p.407-412
Main Authors: Murata, H, Sunagawa, M, Yamazaki, T, Shishido, K, Igasaki, T
Format: Article
Language:English
Subjects:
Agrobacterium tumefaciens
Basidiomycetes
Basidiomycota - genetics
Basidiomycota - metabolism
Biological and medical sciences
Coriolus hirsutus
DNA, Fungal - chemistry
DNA, Fungal - genetics
ectomycorrhizae
Fundamental and applied biological sciences. Psychology
gene expression
gene transfer
genes
genetic transformation
Genetic Vectors - genetics
Genetics
green fluorescent protein
Luminescent Proteins - biosynthesis
Luminescent Proteins - genetics
Luminescent Proteins - metabolism
Microscopy, Fluorescence
Mycorrhizae - genetics
Mycorrhizae - metabolism
mycorrhizal fungi
Parasitism and symbiosis
Plant physiology and development
Polymerase Chain Reaction
Rhizobium - genetics
Suillus
Suillus grevillei
Symbiosis
Transformation, Genetic - physiology
transgenic plants
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Summary:Recombinants were generated from the ectomycorrhizal basidiomycete, Suillus grevillei, through agroinfection using a binary vector carrying the hygromycin B resistance and the autofluorescent protein, DsRed2, markers. DsRed2 was driven by a cis-regulatory region of the glyceraldeyde-3-phosphate dehydrogenase gene (gpd) from the wood-rotting basidiomycete, Coriolus hirsutus, which contains promoters and 5' gpd sequences with first through fourth exons and expressed for the first time in Suillus spp. The transformation system and recombinants expressing an autofluorescent protein may be useful in genetic analysis of the symbiosis.
ISSN:0940-6360
1432-1890
DOI:10.1007/s00572-006-0058-5