Decreased association of aged alpha crystallins with gamma crystallins

Previous studies have demonstrated non-covalent interactions of alpha crystallins with gamma crystallins, under true equilibrium conditions. These interactions could affect short-range interactions of lens crystallins that are necessary for the transparent properties of the lens. Since the transpare...

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Bibliographic Details
Published in:Experimental eye research 2006-10, Vol.83 (4), p.793-797
Main Authors: Takemoto, Larry J., Ponce, Aldo A.
Format: Article
Language:English
Subjects:
aged alpha crystallin
Aging - metabolism
alpha-Crystallins - metabolism
Animals
Cattle
Chromatography, Gel - methods
Fetus - metabolism
gamma crystallin
gamma-Crystallins - metabolism
lens
Lens, Crystalline - embryology
Lens, Crystalline - metabolism
Microdialysis - methods
Protein Binding
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Summary:Previous studies have demonstrated non-covalent interactions of alpha crystallins with gamma crystallins, under true equilibrium conditions. These interactions could affect short-range interactions of lens crystallins that are necessary for the transparent properties of the lens. Since the transparent properties of the lens decrease during aging, it is possible that there are corresponding changes in the ability of aged alpha crystallins to interact with gamma crystallins. In the following study, alpha crystallins were prepared from fetal and aged bovine lenses, then tested for binding to gamma crystallins using microequilibrium dialysis. The results demonstrate that during aging of the normal bovine lens, there is a decrease in the ability of alpha crystallins to bind to gamma crystallins, consistent with the involvement of this interaction in the transparent properties of the lens.
ISSN:0014-4835
1096-0007
DOI:10.1016/j.exer.2006.03.020