Identification of three new bovine T-cell receptor delta variable gene subgroups expressed by peripheral blood T cells

To understand the biology of gammadelta T cells in ruminants, it is necessary to have a comprehensive picture of gammadelta T-cell receptor gene diversity and expression. In this study, three new subgroups of bovine T-cell receptor delta (TRD) variable genes were identified by RT-PCR and sequencing...

Full description

Saved in:
Bibliographic Details
Published in:Immunogenetics (New York) 2006-09, Vol.58 (9), p.746-757
Main Authors: Herzig, Carolyn T A, Blumerman, Seth L, Baldwin, Cynthia L
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Animals
Base Sequence
Cattle - genetics
Cattle - immunology
Chromosome Mapping
Complementarity Determining Regions
DNA - genetics
Gene Expression
Genes
Genes, T-Cell Receptor delta
Humans
Molecular Sequence Data
Phylogeny
Ruminantia
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Sheep
Species Specificity
T cell receptors
T-Lymphocyte Subsets - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To understand the biology of gammadelta T cells in ruminants, it is necessary to have a comprehensive picture of gammadelta T-cell receptor gene diversity and expression. In this study, three new subgroups of bovine T-cell receptor delta (TRD) variable genes were identified by RT-PCR and sequencing and homology with TRDV genes from other mammals determined. Previously unidentified TRDV subgroup genes described in this study include the bovine homologues of ovine TRDV2, TRDV3, and TRDV4 which were named accordingly. TRDV2 subgroup has two genes (TRDV2-1 and TRDV2-2) while we found the previously identified TRDV1 has at least eight genes corresponding to separate genomic sequences. Nucleotide and amino acid sequences for particular gene subgroups between cattle and sheep were more than 87% identical but identities among TRDV subgroups within a species were much less, with bovine TRDV4 having
ISSN:0093-7711
1432-1211
DOI:10.1007/s00251-006-0136-z