Ionic Liquid Matrix-Induced Metastable Decay of Peptides and Oligonucleotides and Stabilization of Phospholipids in MALDI FTMS Analyses

Room-temperature ionic liquid matrices (ILMs) have recently been investigated for use in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) and proven to be advantageous. Literature accounts of ILM performance for biological samples document increased sensitivity and ionizati...

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 2005-12, Vol.16 (12), p.2000-2008
Main Authors: Jones, Jeffrey J., Batoy, S. Mariccor A.B., Wilkins, Charles L., Liyanage, Rohana, Lay, Jackson O.
Format: Article
Language:English
Subjects:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Biological properties
Desorption
Drug Stability
Fragmentation
Fundamental and applied biological sciences. Psychology
Ionic liquids
Ionization
Ions
Lasers
Mass spectrometry
Oligonucleotides
Oligonucleotides - analysis
Oligonucleotides - chemistry
Peptides
Peptides - analysis
Peptides - chemistry
Phospholipids
Phospholipids - analysis
Phospholipids - chemistry
Scientific imaging
Solutions
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Spectroscopy
Spectroscopy, Fourier Transform Infrared - methods
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Summary:Room-temperature ionic liquid matrices (ILMs) have recently been investigated for use in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) and proven to be advantageous. Literature accounts of ILM performance for biological samples document increased sensitivity and ionization efficiency. These claims have been investigated here, and are supported for MALDI TOF applications to peptides, oligonucleotides, and phospholipids. Peptides and oligonucleotides however, do not behave in the same way when ILMs are used for MALDI FTMS. As reported here, with 3 tesla MALDI FTMS peptides and oligonucleotides fragment readily. These observations contrast with those found for MALDI time-of-flight mass spectrometry. Fragmentation is apparently slower than the time required to accelerate ions in a MALDI TOF mass spectrometer, but is readily observed by MALDI FTMS. Therefore, fragmentation of these molecules must occur on a relatively slow time scale. As trapping time is extended, increased fragmentation of peptides and oligonucleotides is seen. However, phospholipids do not fragment extensively. Furthermore, use of traditional solid matrices causes significant fragmentation for this category of compound but is suppressed by use of ILMs.
ISSN:1044-0305
1879-1123
DOI:10.1016/j.jasms.2005.08.007