Differential expression of genes encoding protein kinase CK2 subunits in the plant cell cycle

• Published in: Journal of experimental botany 2005-12, Vol.56 (422), p.3183-3192
• Main Authors: Espunya, M. Carme, López-Giráldez, Trinitat, Hernan, Immaculada, Carballo, Miguel, Martínez, M. Carmen
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; amino acid sequences; Amino acids; Biological and medical sciences; Casein Kinase II - genetics; Casein Kinase II - metabolism; Cell cycle; Cell Cycle - genetics; Cell growth; Cell Line; cell lines; Cell physiology; cell proliferation; CK2α subunit; CK2β subunit; Cloning, Molecular; Complementary DNA; Corn; cultured cells; Drosophila; Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression Profiling; gene expression regulation; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Genes; Messenger RNA; Molecular and cellular biology; Molecular Sequence Data; Nicotiana - cytology; Nicotiana - enzymology; Nicotiana - genetics; Nicotiana tabacum; nucleotide sequences; Phylogeny; plant cell cycle; Plant Proteins - genetics; Plant Proteins - metabolism; polymerase chain reaction; protein kinase CK2; protein structure; protein subunits; Protein Subunits - genetics; Protein Subunits - metabolism; real time-polymerase chain reaction; Research Papers; Reverse transcriptase polymerase chain reaction; RNA; Sequence Alignment; Sequence Analysis, Protein; Sequence Homology, Amino Acid; Signal transduction; tobacco; tobacco BY-2 cells; transcriptional regulation
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jxb/eri315

Protein kinase CK2 is a ubiquitous Ser/Thr/Tyr kinase essential for cell viability in eukaryotes. It comprises α catalytic and β regulatory subunits, which combine to form the classical tetrameric structure, α2β2. Although CK2 is a component of the network that controls the eukaryotic cell cycle, very little is known about the expression patterns of genes encoding its constituent subunits, especially in plants. A study of the complexity of CK2α- and CK2β-encoding genes in BY-2 cells was undertaken in this work, and cloning of the different members of the gene families was performed. The expression of the individual members of each family in relation to cell proliferation was measured by real time RT-PCR. The data obtained provide an accurate understanding of the transcriptional regulation of CK2 in relation to the cell cycle and cell proliferation.