Dual-color split signal fluorescence in situ hybridization assays for the detection of CALM/AF10 in t(10;11)(p13;q14-q21)-positive acute leukemia

Hematology and Bone Marrow Transplantation Unit, Policlinico Monteluce, via Brunamonti, 06122 Perugia, Italy. We developed dual-color split fluorescence in situ hybridization (FISH) assays to detect AF10 and/or CALM rearrangements. Among nine cases of acute leukemia with translocation breakpoints at...

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Bibliographic Details
Published in:Haematologica (Roma) 2006-09, Vol.91 (9), p.1248-1251
Main Authors: La Starza, R, Crescenzi, B, Krause, A, Pierini, V, Specchia, G, Bardi, A, Nieddu, R, Ariola, C, Nanni, M, Diverio, D, Aventin, A, Sborgia, M, Martelli, MF, Bohlander, SK, Mecucci, C
Format: Article
Language:English
Subjects:
Acute Disease
Adolescent
Adult
Biological and medical sciences
Child
Female
Hematologic and hematopoietic diseases
Humans
In Situ Hybridization, Fluorescence - methods
Leukemia - genetics
Leukemia - pathology
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Leukemic Infiltration - diagnosis
Male
Medical sciences
Middle Aged
Oncogene Proteins, Fusion - genetics
Retrospective Studies
Translocation, Genetic
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Summary:Hematology and Bone Marrow Transplantation Unit, Policlinico Monteluce, via Brunamonti, 06122 Perugia, Italy. We developed dual-color split fluorescence in situ hybridization (FISH) assays to detect AF10 and/or CALM rearrangements. Among nine cases of acute leukemia with translocation breakpoints at 10p13 and 11q14-21, a CALM/AF10 rearrangement was found in seven and was confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) in all. In 2/7 cases, FISH detected CALM/AF10 in extramedullary leukemic infiltrations in the mediastinum and breast. As expected, FISH was less sensitive than RT-PCR for disease monitoring of CALM-AF10 positive cases. This new FISH assay reliably discriminates between MLL/AF10 and CALM/AF10 genomic rearrangements, identifies variant and complex CALM/AF10 translocations and detects the CALM/AF10 rearrangement in extramedullary leukemic infiltrations.
ISSN:0390-6078
1592-8721