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Dual-color split signal fluorescence in situ hybridization assays for the detection of CALM/AF10 in t(10;11)(p13;q14-q21)-positive acute leukemia
Hematology and Bone Marrow Transplantation Unit, Policlinico Monteluce, via Brunamonti, 06122 Perugia, Italy. We developed dual-color split fluorescence in situ hybridization (FISH) assays to detect AF10 and/or CALM rearrangements. Among nine cases of acute leukemia with translocation breakpoints at...
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| Published in: | Haematologica (Roma) 2006-09, Vol.91 (9), p.1248-1251 |
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| creator | La Starza, R Crescenzi, B Krause, A Pierini, V Specchia, G Bardi, A Nieddu, R Ariola, C Nanni, M Diverio, D Aventin, A Sborgia, M Martelli, MF Bohlander, SK Mecucci, C |
| description | Hematology and Bone Marrow Transplantation Unit, Policlinico Monteluce, via Brunamonti, 06122 Perugia, Italy.
We developed dual-color split fluorescence in situ hybridization (FISH) assays to detect AF10 and/or CALM rearrangements. Among nine cases of acute leukemia with translocation breakpoints at 10p13 and 11q14-21, a CALM/AF10 rearrangement was found in seven and was confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) in all. In 2/7 cases, FISH detected CALM/AF10 in extramedullary leukemic infiltrations in the mediastinum and breast. As expected, FISH was less sensitive than RT-PCR for disease monitoring of CALM-AF10 positive cases. This new FISH assay reliably discriminates between MLL/AF10 and CALM/AF10 genomic rearrangements, identifies variant and complex CALM/AF10 translocations and detects the CALM/AF10 rearrangement in extramedullary leukemic infiltrations. |
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We developed dual-color split fluorescence in situ hybridization (FISH) assays to detect AF10 and/or CALM rearrangements. Among nine cases of acute leukemia with translocation breakpoints at 10p13 and 11q14-21, a CALM/AF10 rearrangement was found in seven and was confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) in all. In 2/7 cases, FISH detected CALM/AF10 in extramedullary leukemic infiltrations in the mediastinum and breast. As expected, FISH was less sensitive than RT-PCR for disease monitoring of CALM-AF10 positive cases. This new FISH assay reliably discriminates between MLL/AF10 and CALM/AF10 genomic rearrangements, identifies variant and complex CALM/AF10 translocations and detects the CALM/AF10 rearrangement in extramedullary leukemic infiltrations.</description><identifier>ISSN: 0390-6078</identifier><identifier>EISSN: 1592-8721</identifier><identifier>PMID: 16956826</identifier><language>eng</language><publisher>Pavia: Haematologica</publisher><subject>Acute Disease ; Adolescent ; Adult ; Biological and medical sciences ; Child ; Female ; Hematologic and hematopoietic diseases ; Humans ; In Situ Hybridization, Fluorescence - methods ; Leukemia - genetics ; Leukemia - pathology ; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis ; Leukemic Infiltration - diagnosis ; Male ; Medical sciences ; Middle Aged ; Oncogene Proteins, Fusion - genetics ; Retrospective Studies ; Translocation, Genetic</subject><ispartof>Haematologica (Roma), 2006-09, Vol.91 (9), p.1248-1251</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18107733$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16956826$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>La Starza, R</creatorcontrib><creatorcontrib>Crescenzi, B</creatorcontrib><creatorcontrib>Krause, A</creatorcontrib><creatorcontrib>Pierini, V</creatorcontrib><creatorcontrib>Specchia, G</creatorcontrib><creatorcontrib>Bardi, A</creatorcontrib><creatorcontrib>Nieddu, R</creatorcontrib><creatorcontrib>Ariola, C</creatorcontrib><creatorcontrib>Nanni, M</creatorcontrib><creatorcontrib>Diverio, D</creatorcontrib><creatorcontrib>Aventin, A</creatorcontrib><creatorcontrib>Sborgia, M</creatorcontrib><creatorcontrib>Martelli, MF</creatorcontrib><creatorcontrib>Bohlander, SK</creatorcontrib><creatorcontrib>Mecucci, C</creatorcontrib><title>Dual-color split signal fluorescence in situ hybridization assays for the detection of CALM/AF10 in t(10;11)(p13;q14-q21)-positive acute leukemia</title><title>Haematologica (Roma)</title><addtitle>Haematologica</addtitle><description>Hematology and Bone Marrow Transplantation Unit, Policlinico Monteluce, via Brunamonti, 06122 Perugia, Italy.
We developed dual-color split fluorescence in situ hybridization (FISH) assays to detect AF10 and/or CALM rearrangements. Among nine cases of acute leukemia with translocation breakpoints at 10p13 and 11q14-21, a CALM/AF10 rearrangement was found in seven and was confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) in all. In 2/7 cases, FISH detected CALM/AF10 in extramedullary leukemic infiltrations in the mediastinum and breast. As expected, FISH was less sensitive than RT-PCR for disease monitoring of CALM-AF10 positive cases. This new FISH assay reliably discriminates between MLL/AF10 and CALM/AF10 genomic rearrangements, identifies variant and complex CALM/AF10 translocations and detects the CALM/AF10 rearrangement in extramedullary leukemic infiltrations.</description><subject>Acute Disease</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Female</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence - methods</subject><subject>Leukemia - genetics</subject><subject>Leukemia - pathology</subject><subject>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</subject><subject>Leukemic Infiltration - diagnosis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Oncogene Proteins, Fusion - genetics</subject><subject>Retrospective Studies</subject><subject>Translocation, Genetic</subject><issn>0390-6078</issn><issn>1592-8721</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNpFkMlOwzAQhiMEgrK8AvIF1B4ivCWxxakqqwTiAufIScbE4CyNHaryFrwxBop6Gmnm-7_RzE40IYmkscgo2Y0mmEkcpzgTB9Ghc28YUyxlth8dkFQmqaDpJPq6GpWNy852A3K9NR4589oqi7QduwFcCW0JyLSh7UdUr4vBVOZTedO1SDmn1g7pEPU1oAo8lL-DTqPF_OHxYn5D8E_WTwm-JGQ27Qm7XBIeLymZxX0XnOYDkCpHD8jC-A6NUcfRnlbWwcmmHkUvN9fPi7v44en2PmjjmmbYx5wqkDRJUqZZxoFDJZjgFUt0xRlLWUJ4KTGnRCia8aoohAYMRNMiFazCwI6i8z9vP3TLEZzPGxPOtVa10I0uT4VgOMtEAE834Fg0UOX9YBo1rPP_JwbgbAMoVyqrB9WWxm05QYKHse3G2rzWKzNA7hplbdDSfLVaSZLLnFAu2DdjyYdu</recordid><startdate>20060901</startdate><enddate>20060901</enddate><creator>La Starza, R</creator><creator>Crescenzi, B</creator><creator>Krause, A</creator><creator>Pierini, V</creator><creator>Specchia, G</creator><creator>Bardi, A</creator><creator>Nieddu, R</creator><creator>Ariola, C</creator><creator>Nanni, M</creator><creator>Diverio, D</creator><creator>Aventin, A</creator><creator>Sborgia, M</creator><creator>Martelli, MF</creator><creator>Bohlander, SK</creator><creator>Mecucci, C</creator><general>Haematologica</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20060901</creationdate><title>Dual-color split signal fluorescence in situ hybridization assays for the detection of CALM/AF10 in t(10;11)(p13;q14-q21)-positive acute leukemia</title><author>La Starza, R ; Crescenzi, B ; Krause, A ; Pierini, V ; Specchia, G ; Bardi, A ; Nieddu, R ; Ariola, C ; Nanni, M ; Diverio, D ; Aventin, A ; Sborgia, M ; Martelli, MF ; Bohlander, SK ; Mecucci, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h270t-42ae925563f374e4ed8384d35fd43363514c904218a274dbb8fe0e1f2b683d0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Acute Disease</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Female</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence - methods</topic><topic>Leukemia - genetics</topic><topic>Leukemia - pathology</topic><topic>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</topic><topic>Leukemic Infiltration - diagnosis</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Oncogene Proteins, Fusion - genetics</topic><topic>Retrospective Studies</topic><topic>Translocation, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>La Starza, R</creatorcontrib><creatorcontrib>Crescenzi, B</creatorcontrib><creatorcontrib>Krause, A</creatorcontrib><creatorcontrib>Pierini, V</creatorcontrib><creatorcontrib>Specchia, G</creatorcontrib><creatorcontrib>Bardi, A</creatorcontrib><creatorcontrib>Nieddu, R</creatorcontrib><creatorcontrib>Ariola, C</creatorcontrib><creatorcontrib>Nanni, M</creatorcontrib><creatorcontrib>Diverio, D</creatorcontrib><creatorcontrib>Aventin, A</creatorcontrib><creatorcontrib>Sborgia, M</creatorcontrib><creatorcontrib>Martelli, MF</creatorcontrib><creatorcontrib>Bohlander, SK</creatorcontrib><creatorcontrib>Mecucci, C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Haematologica (Roma)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>La Starza, R</au><au>Crescenzi, B</au><au>Krause, A</au><au>Pierini, V</au><au>Specchia, G</au><au>Bardi, A</au><au>Nieddu, R</au><au>Ariola, C</au><au>Nanni, M</au><au>Diverio, D</au><au>Aventin, A</au><au>Sborgia, M</au><au>Martelli, MF</au><au>Bohlander, SK</au><au>Mecucci, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dual-color split signal fluorescence in situ hybridization assays for the detection of CALM/AF10 in t(10;11)(p13;q14-q21)-positive acute leukemia</atitle><jtitle>Haematologica (Roma)</jtitle><addtitle>Haematologica</addtitle><date>2006-09-01</date><risdate>2006</risdate><volume>91</volume><issue>9</issue><spage>1248</spage><epage>1251</epage><pages>1248-1251</pages><issn>0390-6078</issn><eissn>1592-8721</eissn><abstract>Hematology and Bone Marrow Transplantation Unit, Policlinico Monteluce, via Brunamonti, 06122 Perugia, Italy.
We developed dual-color split fluorescence in situ hybridization (FISH) assays to detect AF10 and/or CALM rearrangements. Among nine cases of acute leukemia with translocation breakpoints at 10p13 and 11q14-21, a CALM/AF10 rearrangement was found in seven and was confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) in all. In 2/7 cases, FISH detected CALM/AF10 in extramedullary leukemic infiltrations in the mediastinum and breast. As expected, FISH was less sensitive than RT-PCR for disease monitoring of CALM-AF10 positive cases. This new FISH assay reliably discriminates between MLL/AF10 and CALM/AF10 genomic rearrangements, identifies variant and complex CALM/AF10 translocations and detects the CALM/AF10 rearrangement in extramedullary leukemic infiltrations.</abstract><cop>Pavia</cop><pub>Haematologica</pub><pmid>16956826</pmid><tpages>4</tpages></addata></record> |
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| ispartof | Haematologica (Roma), 2006-09, Vol.91 (9), p.1248-1251 |
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| source | Freely Accessible Science Journals - check A-Z of ejournals |
| subjects | Acute Disease Adolescent Adult Biological and medical sciences Child Female Hematologic and hematopoietic diseases Humans In Situ Hybridization, Fluorescence - methods Leukemia - genetics Leukemia - pathology Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Leukemic Infiltration - diagnosis Male Medical sciences Middle Aged Oncogene Proteins, Fusion - genetics Retrospective Studies Translocation, Genetic |
| title | Dual-color split signal fluorescence in situ hybridization assays for the detection of CALM/AF10 in t(10;11)(p13;q14-q21)-positive acute leukemia |
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