Effects of Human Breast Stromal Cells on Conjugated Linoleic Acid (CLA) Modulated Vascular Endothelial Growth Factor-A (VEGF-A) Expression in MCF-7 Cells

Background: Conjugated linoleic acid (CLA), a naturally occurring compound found in ruminant dairy and beef products, has been shown to possess anti-cancer ability in vivo and in vitro. There are several CLA isomers in ruminant-produced food products, among which t10,c12-CLA and c9,t11-CLA are most...

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Bibliographic Details
Published in:Anticancer research 2005-11, Vol.25 (6B), p.4061-4068
Main Authors: Wang, Li-Shu, Huang, Yi-Wen, Sugimoto, Yasuro, Liu, Suling, Chang, Hsiang-Lin, Ye, Weiping, Shu, Sherry, Lin, Young C
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biomarkers, Tumor - biosynthesis
Biomarkers, Tumor - genetics
Breast Neoplasms - blood supply
Breast Neoplasms - drug therapy
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Cell Communication - physiology
Cell Line, Tumor
Coculture Techniques
Down-Regulation
Gynecology. Andrology. Obstetrics
Humans
Isomerism
Linoleic Acids, Conjugated - pharmacology
Mammary gland diseases
Medical sciences
Neovascularization, Pathologic - drug therapy
Neovascularization, Pathologic - genetics
Neovascularization, Pathologic - metabolism
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Stromal Cells - cytology
Tumors
Vascular Endothelial Growth Factor A - biosynthesis
Vascular Endothelial Growth Factor A - genetics
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Summary:Background: Conjugated linoleic acid (CLA), a naturally occurring compound found in ruminant dairy and beef products, has been shown to possess anti-cancer ability in vivo and in vitro. There are several CLA isomers in ruminant-produced food products, among which t10,c12-CLA and c9,t11-CLA are most potent. Vascular endothelial growth factor-A (VEGF-A) has been implicated as an angiogenesis-activating cytokine. Our previous results indicated that CLA induced suppression of VEGF-A in MCF-7 cells, which may be one of CLA's anticancer mechanisms. Materials and Methods: The effects of t10,c12-CLA and c9,t11-CLA on VEGF-A mRNA and protein expression in MCF-7 cells, which were co-cultured with human breast stromal cells isolated from breast tissues of surgical specimens of mammoplasty and breast cancer patients, were detected by RT-PCR and Western blot analysis. Results: VEGF-A mRNA and protein expressions were significantly (p
ISSN:0250-7005
1791-7530