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Effects of Human Breast Stromal Cells on Conjugated Linoleic Acid (CLA) Modulated Vascular Endothelial Growth Factor-A (VEGF-A) Expression in MCF-7 Cells
Background: Conjugated linoleic acid (CLA), a naturally occurring compound found in ruminant dairy and beef products, has been shown to possess anti-cancer ability in vivo and in vitro. There are several CLA isomers in ruminant-produced food products, among which t10,c12-CLA and c9,t11-CLA are most...
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| Published in: | Anticancer research 2005-11, Vol.25 (6B), p.4061-4068 |
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| container_title | Anticancer research |
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| creator | Wang, Li-Shu Huang, Yi-Wen Sugimoto, Yasuro Liu, Suling Chang, Hsiang-Lin Ye, Weiping Shu, Sherry Lin, Young C |
| description | Background: Conjugated linoleic acid (CLA), a naturally occurring compound found in ruminant dairy and beef products, has
been shown to possess anti-cancer ability in vivo and in vitro. There are several CLA isomers in ruminant-produced food products,
among which t10,c12-CLA and c9,t11-CLA are most potent. Vascular endothelial growth factor-A (VEGF-A) has been implicated
as an angiogenesis-activating cytokine. Our previous results indicated that CLA induced suppression of VEGF-A in MCF-7 cells,
which may be one of CLA's anticancer mechanisms. Materials and Methods: The effects of t10,c12-CLA and c9,t11-CLA on VEGF-A
mRNA and protein expression in MCF-7 cells, which were co-cultured with human breast stromal cells isolated from breast tissues
of surgical specimens of mammoplasty and breast cancer patients, were detected by RT-PCR and Western blot analysis. Results:
VEGF-A mRNA and protein expressions were significantly (p |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_68831404</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>68831404</sourcerecordid><originalsourceid>FETCH-LOGICAL-h270t-e21cff45b754a41489cf376e9e0097296380fb4e5a0a6c9b9951a4c394a8fd0f3</originalsourceid><addsrcrecordid>eNpFkMFq3DAQhk1paTZpX6HokpIcDJIlS9ZxY7ybwIYe2uZqZmUpVpCtjSSz6aP0bSuSLWEOMzDf_P_wfyhWREhSiprij8UKVzUuBcb1WXEe4xPGnMuGfi7OCKekwoyvir-dMVqliLxBt8sEM7oJGmJCP1PwEzjUaufydkatn5-WR0h6QDs7e6etQmtlB3TV7tbX6N4Pi3vdPkBUeQyomwefRu1sltkGf0wj2oBKPpRrdPXQbTdlvuteDkHHaLODndF9uynFm-eX4pMBF_XXU78ofm-6X-1tufuxvWvXu3KsBE6lrogyhtV7UTNghDVSGSq4lhpjKSrJaYPNnukaMHAl91LWBJiikkFjBmzoRfH9TfcQ_POiY-onG1X-AGbtl9jzpqGEYZbBbydw2U966A_BThD-9P_DzMDlCcgJgDMBZmXjOyeqXBS_O472cTzaoPuYk3ZZlvYQqrrnNz3DnNB_X1qJlg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>68831404</pqid></control><display><type>article</type><title>Effects of Human Breast Stromal Cells on Conjugated Linoleic Acid (CLA) Modulated Vascular Endothelial Growth Factor-A (VEGF-A) Expression in MCF-7 Cells</title><source>EZB Electronic Journals Library</source><creator>Wang, Li-Shu ; Huang, Yi-Wen ; Sugimoto, Yasuro ; Liu, Suling ; Chang, Hsiang-Lin ; Ye, Weiping ; Shu, Sherry ; Lin, Young C</creator><creatorcontrib>Wang, Li-Shu ; Huang, Yi-Wen ; Sugimoto, Yasuro ; Liu, Suling ; Chang, Hsiang-Lin ; Ye, Weiping ; Shu, Sherry ; Lin, Young C</creatorcontrib><description>Background: Conjugated linoleic acid (CLA), a naturally occurring compound found in ruminant dairy and beef products, has
been shown to possess anti-cancer ability in vivo and in vitro. There are several CLA isomers in ruminant-produced food products,
among which t10,c12-CLA and c9,t11-CLA are most potent. Vascular endothelial growth factor-A (VEGF-A) has been implicated
as an angiogenesis-activating cytokine. Our previous results indicated that CLA induced suppression of VEGF-A in MCF-7 cells,
which may be one of CLA's anticancer mechanisms. Materials and Methods: The effects of t10,c12-CLA and c9,t11-CLA on VEGF-A
mRNA and protein expression in MCF-7 cells, which were co-cultured with human breast stromal cells isolated from breast tissues
of surgical specimens of mammoplasty and breast cancer patients, were detected by RT-PCR and Western blot analysis. Results:
VEGF-A mRNA and protein expressions were significantly (p<0.05) elevated in co-cultured MCF-7 cells in comparison with cultured
MCF-7 cells alone. Normal human breast stromal cells contribute greater effects in increasing VEGF-A protein expression in
MCF-7 cells. Both t10,c12-CLA and c9,t11-CLA significantly (p<0.05) decreased VEGF-A mRNA and protein levels in co-cultured
MCF-7 cells. t10,c12-CLA appeared to be the more active isomer than c9,t11-CLA. Conclusion: The results indicate that dietary
CLA might serve as a chemo-therapeutic agent in human breast cancers by down-regulating VEGF-A expression.</description><identifier>ISSN: 0250-7005</identifier><identifier>EISSN: 1791-7530</identifier><identifier>PMID: 16312046</identifier><language>eng</language><publisher>Attiki: International Institute of Anticancer Research</publisher><subject>Biological and medical sciences ; Biomarkers, Tumor - biosynthesis ; Biomarkers, Tumor - genetics ; Breast Neoplasms - blood supply ; Breast Neoplasms - drug therapy ; Breast Neoplasms - metabolism ; Breast Neoplasms - pathology ; Cell Communication - physiology ; Cell Line, Tumor ; Coculture Techniques ; Down-Regulation ; Gynecology. Andrology. Obstetrics ; Humans ; Isomerism ; Linoleic Acids, Conjugated - pharmacology ; Mammary gland diseases ; Medical sciences ; Neovascularization, Pathologic - drug therapy ; Neovascularization, Pathologic - genetics ; Neovascularization, Pathologic - metabolism ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics ; Stromal Cells - cytology ; Tumors ; Vascular Endothelial Growth Factor A - biosynthesis ; Vascular Endothelial Growth Factor A - genetics</subject><ispartof>Anticancer research, 2005-11, Vol.25 (6B), p.4061-4068</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17272730$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16312046$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Li-Shu</creatorcontrib><creatorcontrib>Huang, Yi-Wen</creatorcontrib><creatorcontrib>Sugimoto, Yasuro</creatorcontrib><creatorcontrib>Liu, Suling</creatorcontrib><creatorcontrib>Chang, Hsiang-Lin</creatorcontrib><creatorcontrib>Ye, Weiping</creatorcontrib><creatorcontrib>Shu, Sherry</creatorcontrib><creatorcontrib>Lin, Young C</creatorcontrib><title>Effects of Human Breast Stromal Cells on Conjugated Linoleic Acid (CLA) Modulated Vascular Endothelial Growth Factor-A (VEGF-A) Expression in MCF-7 Cells</title><title>Anticancer research</title><addtitle>Anticancer Res</addtitle><description>Background: Conjugated linoleic acid (CLA), a naturally occurring compound found in ruminant dairy and beef products, has
been shown to possess anti-cancer ability in vivo and in vitro. There are several CLA isomers in ruminant-produced food products,
among which t10,c12-CLA and c9,t11-CLA are most potent. Vascular endothelial growth factor-A (VEGF-A) has been implicated
as an angiogenesis-activating cytokine. Our previous results indicated that CLA induced suppression of VEGF-A in MCF-7 cells,
which may be one of CLA's anticancer mechanisms. Materials and Methods: The effects of t10,c12-CLA and c9,t11-CLA on VEGF-A
mRNA and protein expression in MCF-7 cells, which were co-cultured with human breast stromal cells isolated from breast tissues
of surgical specimens of mammoplasty and breast cancer patients, were detected by RT-PCR and Western blot analysis. Results:
VEGF-A mRNA and protein expressions were significantly (p<0.05) elevated in co-cultured MCF-7 cells in comparison with cultured
MCF-7 cells alone. Normal human breast stromal cells contribute greater effects in increasing VEGF-A protein expression in
MCF-7 cells. Both t10,c12-CLA and c9,t11-CLA significantly (p<0.05) decreased VEGF-A mRNA and protein levels in co-cultured
MCF-7 cells. t10,c12-CLA appeared to be the more active isomer than c9,t11-CLA. Conclusion: The results indicate that dietary
CLA might serve as a chemo-therapeutic agent in human breast cancers by down-regulating VEGF-A expression.</description><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor - biosynthesis</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Breast Neoplasms - blood supply</subject><subject>Breast Neoplasms - drug therapy</subject><subject>Breast Neoplasms - metabolism</subject><subject>Breast Neoplasms - pathology</subject><subject>Cell Communication - physiology</subject><subject>Cell Line, Tumor</subject><subject>Coculture Techniques</subject><subject>Down-Regulation</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Isomerism</subject><subject>Linoleic Acids, Conjugated - pharmacology</subject><subject>Mammary gland diseases</subject><subject>Medical sciences</subject><subject>Neovascularization, Pathologic - drug therapy</subject><subject>Neovascularization, Pathologic - genetics</subject><subject>Neovascularization, Pathologic - metabolism</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>Stromal Cells - cytology</subject><subject>Tumors</subject><subject>Vascular Endothelial Growth Factor A - biosynthesis</subject><subject>Vascular Endothelial Growth Factor A - genetics</subject><issn>0250-7005</issn><issn>1791-7530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNpFkMFq3DAQhk1paTZpX6HokpIcDJIlS9ZxY7ybwIYe2uZqZmUpVpCtjSSz6aP0bSuSLWEOMzDf_P_wfyhWREhSiprij8UKVzUuBcb1WXEe4xPGnMuGfi7OCKekwoyvir-dMVqliLxBt8sEM7oJGmJCP1PwEzjUaufydkatn5-WR0h6QDs7e6etQmtlB3TV7tbX6N4Pi3vdPkBUeQyomwefRu1sltkGf0wj2oBKPpRrdPXQbTdlvuteDkHHaLODndF9uynFm-eX4pMBF_XXU78ofm-6X-1tufuxvWvXu3KsBE6lrogyhtV7UTNghDVSGSq4lhpjKSrJaYPNnukaMHAl91LWBJiikkFjBmzoRfH9TfcQ_POiY-onG1X-AGbtl9jzpqGEYZbBbydw2U966A_BThD-9P_DzMDlCcgJgDMBZmXjOyeqXBS_O472cTzaoPuYk3ZZlvYQqrrnNz3DnNB_X1qJlg</recordid><startdate>20051101</startdate><enddate>20051101</enddate><creator>Wang, Li-Shu</creator><creator>Huang, Yi-Wen</creator><creator>Sugimoto, Yasuro</creator><creator>Liu, Suling</creator><creator>Chang, Hsiang-Lin</creator><creator>Ye, Weiping</creator><creator>Shu, Sherry</creator><creator>Lin, Young C</creator><general>International Institute of Anticancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20051101</creationdate><title>Effects of Human Breast Stromal Cells on Conjugated Linoleic Acid (CLA) Modulated Vascular Endothelial Growth Factor-A (VEGF-A) Expression in MCF-7 Cells</title><author>Wang, Li-Shu ; Huang, Yi-Wen ; Sugimoto, Yasuro ; Liu, Suling ; Chang, Hsiang-Lin ; Ye, Weiping ; Shu, Sherry ; Lin, Young C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h270t-e21cff45b754a41489cf376e9e0097296380fb4e5a0a6c9b9951a4c394a8fd0f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor - biosynthesis</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Breast Neoplasms - blood supply</topic><topic>Breast Neoplasms - drug therapy</topic><topic>Breast Neoplasms - metabolism</topic><topic>Breast Neoplasms - pathology</topic><topic>Cell Communication - physiology</topic><topic>Cell Line, Tumor</topic><topic>Coculture Techniques</topic><topic>Down-Regulation</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Isomerism</topic><topic>Linoleic Acids, Conjugated - pharmacology</topic><topic>Mammary gland diseases</topic><topic>Medical sciences</topic><topic>Neovascularization, Pathologic - drug therapy</topic><topic>Neovascularization, Pathologic - genetics</topic><topic>Neovascularization, Pathologic - metabolism</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><topic>Stromal Cells - cytology</topic><topic>Tumors</topic><topic>Vascular Endothelial Growth Factor A - biosynthesis</topic><topic>Vascular Endothelial Growth Factor A - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Li-Shu</creatorcontrib><creatorcontrib>Huang, Yi-Wen</creatorcontrib><creatorcontrib>Sugimoto, Yasuro</creatorcontrib><creatorcontrib>Liu, Suling</creatorcontrib><creatorcontrib>Chang, Hsiang-Lin</creatorcontrib><creatorcontrib>Ye, Weiping</creatorcontrib><creatorcontrib>Shu, Sherry</creatorcontrib><creatorcontrib>Lin, Young C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Anticancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Li-Shu</au><au>Huang, Yi-Wen</au><au>Sugimoto, Yasuro</au><au>Liu, Suling</au><au>Chang, Hsiang-Lin</au><au>Ye, Weiping</au><au>Shu, Sherry</au><au>Lin, Young C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Human Breast Stromal Cells on Conjugated Linoleic Acid (CLA) Modulated Vascular Endothelial Growth Factor-A (VEGF-A) Expression in MCF-7 Cells</atitle><jtitle>Anticancer research</jtitle><addtitle>Anticancer Res</addtitle><date>2005-11-01</date><risdate>2005</risdate><volume>25</volume><issue>6B</issue><spage>4061</spage><epage>4068</epage><pages>4061-4068</pages><issn>0250-7005</issn><eissn>1791-7530</eissn><abstract>Background: Conjugated linoleic acid (CLA), a naturally occurring compound found in ruminant dairy and beef products, has
been shown to possess anti-cancer ability in vivo and in vitro. There are several CLA isomers in ruminant-produced food products,
among which t10,c12-CLA and c9,t11-CLA are most potent. Vascular endothelial growth factor-A (VEGF-A) has been implicated
as an angiogenesis-activating cytokine. Our previous results indicated that CLA induced suppression of VEGF-A in MCF-7 cells,
which may be one of CLA's anticancer mechanisms. Materials and Methods: The effects of t10,c12-CLA and c9,t11-CLA on VEGF-A
mRNA and protein expression in MCF-7 cells, which were co-cultured with human breast stromal cells isolated from breast tissues
of surgical specimens of mammoplasty and breast cancer patients, were detected by RT-PCR and Western blot analysis. Results:
VEGF-A mRNA and protein expressions were significantly (p<0.05) elevated in co-cultured MCF-7 cells in comparison with cultured
MCF-7 cells alone. Normal human breast stromal cells contribute greater effects in increasing VEGF-A protein expression in
MCF-7 cells. Both t10,c12-CLA and c9,t11-CLA significantly (p<0.05) decreased VEGF-A mRNA and protein levels in co-cultured
MCF-7 cells. t10,c12-CLA appeared to be the more active isomer than c9,t11-CLA. Conclusion: The results indicate that dietary
CLA might serve as a chemo-therapeutic agent in human breast cancers by down-regulating VEGF-A expression.</abstract><cop>Attiki</cop><pub>International Institute of Anticancer Research</pub><pmid>16312046</pmid><tpages>8</tpages></addata></record> |
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| subjects | Biological and medical sciences Biomarkers, Tumor - biosynthesis Biomarkers, Tumor - genetics Breast Neoplasms - blood supply Breast Neoplasms - drug therapy Breast Neoplasms - metabolism Breast Neoplasms - pathology Cell Communication - physiology Cell Line, Tumor Coculture Techniques Down-Regulation Gynecology. Andrology. Obstetrics Humans Isomerism Linoleic Acids, Conjugated - pharmacology Mammary gland diseases Medical sciences Neovascularization, Pathologic - drug therapy Neovascularization, Pathologic - genetics Neovascularization, Pathologic - metabolism RNA, Messenger - biosynthesis RNA, Messenger - genetics Stromal Cells - cytology Tumors Vascular Endothelial Growth Factor A - biosynthesis Vascular Endothelial Growth Factor A - genetics |
| title | Effects of Human Breast Stromal Cells on Conjugated Linoleic Acid (CLA) Modulated Vascular Endothelial Growth Factor-A (VEGF-A) Expression in MCF-7 Cells |
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