Mitochondrial dysfunction enhances Gal4-dependent transcription

Mitochondrial dysfunction has been shown to elicit broad effects on nuclear gene expression. We show here that transcription dependent on the prototypical acidic activator Gal4 is responsive to mitochondrial dysfunction. In cells with no mitochondrial DNA, Gal4-dependent gene expression is elevated....

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Bibliographic Details
Published in:FEMS microbiology letters 2005-12, Vol.253 (2), p.207-213
Main Authors: Jeličić, Branka, Traven, Ana, Filić, Vedrana, Sopta, Mary
Format: Article
Language:English
Subjects:
Binding
Deoxyribonucleic acid
DNA
DNA, Bacterial - metabolism
DNA-Binding Proteins
Electrophoretic mobility
Fermentation
Gal4
Galactose
Galactose - metabolism
Gene expression
Gene Expression Regulation, Fungal
Microbiology
Mitochondria
Mitochondria - metabolism
Mitochondria - pathology
Mitochondrial DNA
Mitochondrial dysfunction
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - metabolism
Transcription
Transcription Factors - metabolism
Transcription, Genetic
Transcriptional Activation - genetics
Up-Regulation
Yeast
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Summary:Mitochondrial dysfunction has been shown to elicit broad effects on nuclear gene expression. We show here that transcription dependent on the prototypical acidic activator Gal4 is responsive to mitochondrial dysfunction. In cells with no mitochondrial DNA, Gal4-dependent gene expression is elevated. A minimal Gal4 activator containing the DNA binding and activation domain is sufficient for this response. Transcription dependent on a fusion of Gal4 to a heterologous DNA binding domain is similarly elevated in a mitochondrial mutant. Analysis of different Gal4-dependent promoters and gel mobility shift assays suggest that the effect of mitochondrial dysfunction on Gal4 activity is related to increased DNA binding to the cognate Gal4 element. Given that fermentation is the only means to obtain energy in respiratory deficient cells, it is possible that higher Gal4 activity in cells with dysfunctional mitochondria works to promote more efficient fermentation of galactose.
ISSN:0378-1097
1574-6968
DOI:10.1016/j.femsle.2005.09.033