The heme-binding lipoprotein (HbpA) of Haemophilus influenzae: Role in heme utilization

Haemophilus influenzae has an absolute growth requirement for heme and a heme binding lipoprotein (HbpA) has been implicated in the utilization of this essential nutrient. HbpA was identified by examining clones from an H. influenzae genomic library that caused Escherichia coli harboring the clone t...

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Bibliographic Details
Published in:FEMS microbiology letters 2005-12, Vol.253 (2), p.193-199
Main Authors: Morton, Daniel J., Madore, Larissa L., Smith, Ann, VanWagoner, Timothy M., Seale, Thomas W., Whitby, Paul W., Stull, Terrence L.
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacteriology
Binding
Biological and medical sciences
Carrier Proteins - genetics
Carrier Proteins - metabolism
Cytosol - metabolism
E coli
Escherichia coli
Fundamental and applied biological sciences. Psychology
Gene Rearrangement
Genetics
Haemophilus influenzae
Haemophilus influenzae - genetics
Haemophilus influenzae - growth & development
Haemophilus influenzae - metabolism
Heme
Heme - metabolism
Heme-binding protein
Lipoproteins - genetics
Lipoproteins - metabolism
Metabolism. Enzymes
Microbiology
Mutation
Nutrient requirements
Nutrient utilization
Utilization
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Summary:Haemophilus influenzae has an absolute growth requirement for heme and a heme binding lipoprotein (HbpA) has been implicated in the utilization of this essential nutrient. HbpA was identified by examining clones from an H. influenzae genomic library that caused Escherichia coli harboring the clone to bind heme. However, HbpA has not been shown to mediate heme acquisition in H. influenzae. We constructed an insertional mutation of hbpA in a nontypeable H. influenzae strain and demonstrated a role for the gene in utilization of multiple heme sources. This is the first report confirming a role for HbpA in utilization of heme.
ISSN:0378-1097
1574-6968
DOI:10.1016/j.femsle.2005.09.016